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https://hdl.handle.net/1822/50469
Título: | Toxic reagents and expensive equipment: are they really necessary for the extraction of good quality fungal DNA? |
Autor(es): | Rodrigues, P. Venâncio, Armando Lima, Nelson |
Palavras-chave: | Genomics DNA extraction fungi PCR Aspergillus |
Data: | 2018 |
Editora: | Wiley-Blackwell |
Revista: | Letters in Applied Microbiology |
Citação: | Rodrigues, P.; Venâncio, Armando; Lima, Nelson, Toxic reagents and expensive equipment: are they really necessary for the extraction of good quality fungal DNA?. Letters in Applied Microbiology, 66(1), 32-37, 2018 |
Resumo(s): | The aim of this work was to evaluate a fungal DNA extraction procedure with the lowest inputs in terms of time as well as of expensive and toxic chemicals, but able to consistently produce genomic DNA of good quality for PCR purposes. Two types of fungal biological material were tested - mycelium and conidia - combined with two protocols for DNA extraction using Sodium Dodecyl Sulphate [SDS] and Cetyl Trimethyl Ammonium Bromide [CTAB] as extraction buffers and glass beads for mechanical disruption of cell walls. Our results showed that conidia and SDS buffer was the combination that lead to the best DNA quality and yield, with the lowest variation between samples. This study clearly demonstrates that it is possible to obtain high yield and pure DNA from pigmented conidia without the use of strong cell disrupting procedures and of toxic reagents. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/50469 |
DOI: | 10.1111/lam.12822 |
ISSN: | 0266-8254 |
e-ISSN: | 1472-765X |
Versão da editora: | http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1472-765X |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_47183_1.pdf | 332,97 kB | Adobe PDF | Ver/Abrir |