Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/49479
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Silvério, Sara Isabel Cruz | por |
dc.contributor.author | Macedo, Eugénia A. | por |
dc.contributor.author | Teixeira, J. A. | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.date.accessioned | 2018-01-19T18:07:00Z | - |
dc.date.available | 2018-01-19T18:07:00Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.citation | Silvério, Sara C.; Macedo, Eugénia A.; Teixeira, José A.; Rodrigues, Lígia R., New ß-galactosidase producers with potential for prebiotic synthesis. Bioresource Technology, 250, 131-139, 2018 | por |
dc.identifier.issn | 0960-8524 | por |
dc.identifier.uri | https://hdl.handle.net/1822/49479 | - |
dc.description.abstract | ß-galactosidases (EC 3.2.1.23) are interesting enzymes with potential application in the pharmaceutical and food industry. In this work, a screening study was carried out to identify new fungal sources of -galactosidase. A total of 50 fungi were evaluated using a chromogenic test performed in agar plates. The most promising microorganisms were validated as effective -galactosidase producers under submerged fermentation conditions. The crude -galactosidases were characterized regarding their optimal pH (3.0-5.5) and temperature (45-65 °C). All enzymes showed ability to synthesize lactose-based prebiotics, namely lactulose (maximal yield 3.3%) and a galacto-oligosaccharide (GOS) (maximal yield 20%). Additionally, some enzymatic extracts with fructosyltransferase activity allowed to produce other type of prebiotics, namely fructo-oligosaccharides (FOS). This work, reports for the first time the simultaneous synthesis of different mixtures of GOS (2-15% yield and 0.07-0.5 g/L·h-1 productivity) and FOS (4-30% yield and 0.1-1 g/L·h-1 productivity) by crude extracts exhibiting dual enzymatic activity. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/ BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER006684),and also by the Project BioTecNorte(NORTE-01-0145-FEDER000004) and the project MultiBiorefinery (POCI-01-0145-FEDER016403) funded by the European Regional Development Fund under thescope ofNorte2020 –Programa OperacionalRegional doNorte.SCS acknowledges her post doc grant (SFRH/BPD/88584/2012) from FCT. EA Macedo is a member of the Associate Laboratory LSRE-LCM, Project POCI-01-0145-FEDER-006984, funded by FEDER through COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT – Fundação para a Ciência e a Tecnologia. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F88584%2F2012/PT | por |
dc.rights | openAccess | por |
dc.subject | ß-galactosidase | por |
dc.subject | fungal strains | por |
dc.subject | lactulose | por |
dc.subject | galacto-oligosaccharides | por |
dc.subject | fructo-oligosaccharides | por |
dc.subject | β-Galactosidase | por |
dc.title | New ß-galactosidase producers with potential for prebiotic synthesis | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/bioresource-technology/ | por |
dc.comments | CEB47252 | por |
oaire.citationStartPage | 131 | por |
oaire.citationEndPage | 139 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationVolume | 250 | por |
dc.date.updated | 2018-01-03T23:41:58Z | - |
dc.identifier.eissn | 0960-8524 | por |
dc.identifier.doi | 10.1016/j.biortech.2017.11.045 | por |
dc.identifier.pmid | 29161572 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Bioresource Technology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_47252_1.pdf | 358,46 kB | Adobe PDF | Ver/Abrir |