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https://hdl.handle.net/1822/48148
Título: | Crosstalk between adipose stem cells and tendon cells reveals a temporal regulation of tenogenesis by matrix deposition and remodeling |
Autor(es): | Almeida, Raquel Costa Calejo, Isabel Reis, R. L. Gomes, Manuela E. |
Palavras-chave: | Adipose-derived stem cells Co-culture Extracellular matrix Matrix metalloproteinases Temporal gene regulation Tendon Regeneration Tenogenesis |
Data: | 2018 |
Editora: | Wiley |
Revista: | Journal of Cellular Physiology |
Citação: | Costa-Almeida R., Calejo I., Reis R. L., Gomes M. E. Crosstalk between adipose stem cells and tendon cells reveals a temporal regulation of tenogenesis by matrix deposition and remodeling, Journal Of Cellular Physiology, doi:10.1002/jcp.26363, 2017 |
Resumo(s): | Tendon injuries constitute an unmet clinical challenge owing to the limited intrinsic regenerative ability of this tissue. Cell-based therapies aim at improving tendon healing through the delicate orchestration of tissue rebuilding and regain of function. Hence, human adipose-derived stem cells (hASCs) have been proposed as a promising cell source for boosting tendon regeneration. In this work, we investigated the influence of hASCs on native human tendon-derived cells (hTDCs) through the establishment of a direct contact co-culture system. Results demonstrated that direct interactions between these cell types resulted in controlled proliferation and spontaneous cell elongation. ECM-related genes, particularly COL1A1 and TNC, and genes involved in ECM remodeling, such as MMP1, MMP2, MMP3 and TIMP1, were expressed in co-cultures in a temporally regulated manner. In addition, deposition of collagen type I was accelerated in co-cultures systems and favored over the production of collagen type III, resulting in an enhanced COL1/COL3 ratio as soon as 7 days. In conclusion, hASCs seem to be good candidates in modulating the behavior of native tendon cells, particularly through a balanced process of ECM synthesis and degradation. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/48148 |
DOI: | 10.1002/jcp.26363 |
ISSN: | 0021-9541 |
e-ISSN: | 1097-4652 |
Versão da editora: | http://onlinelibrary.wiley.com/doi/10.1002/jcp.26363/full |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19266-Costa-Almeida_et_al-2017-Journal_of_Cellular_Physiology.pdf Acesso restrito! | 2,15 MB | Adobe PDF | Ver/Abrir |