Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/47926

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dc.contributor.authorSilva, J. M.por
dc.contributor.authorClementino, E. L.por
dc.contributor.authorSouza, K. P. S.por
dc.contributor.authorSales, Amanda Emmanuellepor
dc.contributor.authorNascimento, Thiago Pajeúpor
dc.contributor.authorTeixeira, J. A.por
dc.contributor.authorPorto, Tatiana Souzapor
dc.contributor.authorPorto, Ana Lúcia Figueiredopor
dc.contributor.authorPorto, Camila Souzapor
dc.date.accessioned2017-11-30T14:18:00Z-
dc.date.available2017-11-30T14:18:00Z-
dc.date.issued2012-12-05-
dc.identifier.citationSilva, J. M.; Clementino, E. L.; Souza, K. P. S.; Sales, A. E.; Nascimento, T. P.; Teixeira, José A.; Porto, T. S.; Porto, A. L. F.; Porto, C. S., Evaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573. XI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and Biotechnology. Recife, Brazil, Dec 5-7, 2012.por
dc.identifier.urihttps://hdl.handle.net/1822/47926-
dc.descriptionXI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and Biotechnologypor
dc.description.abstractFibrinolytic enzymes have the ability to degrade fibrin clots formed for avoiding intravascular thrombosis. In the pharmaceutical industry there is a search for new fibrinolytic agent that reduces the production cost and increasing productivity. The use of microorganism for enzyme production, such as the genus Streptomyces has been reported. Streptomyces is a Gram-positive bacteria, responsible for producing many bioactive compounds and extracellular enzymes of pharmaceutical interest. This study aimed to evaluated the production of fibrinolytic protease by Streptomyces sp DPUA 1573. Microbial cells were cultivated in the ISP2 for 48 hours, after this period the strains were inoculated in MS2 (soybean medium) that according with factorial design 24 (concentrations of soybean 0.5; 1.0 and 1.5%, glucose 0; 0.5 and 1.0% and different speeds 150 rpm; 200 rpm and 250 rpm and temperature 28C; 30C and 32C). The factorial design was analyzed by variance analysis (anova) and the glucose concentration showed a positive and significative effect. The results showed that the variable interaction had significant effect. that the best condition was composed 1.5% soybean, 1% glucose, 28 ºC and 150 speed in 48 hours, with production fibrinolytic 1391.66 U/mL. These values were higher than those reported in the literature. However these results show the biggest potencial in production fibrinolytic enzyme by Streptomyces.por
dc.language.isoengpor
dc.rightsopenAccesspor
dc.subjectStreptomycespor
dc.subjectFibrinolytic enzymepor
dc.subjectIntravascular thrombosispor
dc.titleEvaluation the best condition of Fibrinolytic protease production using factorial design by Streptomyces sp DPUA 1573por
dc.typeconferenceAbstractpor
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.sbbq.org.br/v2/index.php?option=com_content&task=view&id=746por
dc.commentsCEB46901por
oaire.citationConferenceDate05 - 07 Dec. 2012por
sdum.event.titleXI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and Biotechnologypor
sdum.event.typemeetingpor
oaire.citationConferencePlaceRecife, Brazilpor
dc.date.updated2017-08-02T16:34:43Z-
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersionpor
Aparece nas coleções:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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