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https://hdl.handle.net/1822/47629
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Campo DC | Valor | Idioma |
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dc.contributor.author | Sá-Pessoa, Joana | por |
dc.contributor.author | Amillis, Sotiris | por |
dc.contributor.author | Casal, Margarida | por |
dc.contributor.author | Diallinas, George | por |
dc.date.accessioned | 2017-11-21T18:11:14Z | - |
dc.date.issued | 2015-03 | - |
dc.identifier.issn | 1087-1845 | por |
dc.identifier.uri | https://hdl.handle.net/1822/47629 | - |
dc.description.abstract | AcpA has been previously characterized as a high-affinity transporter essential for the uptake and use of acetate as sole carbon source in Aspergillus nidulans. Here, we follow the expression profile of AcpA and define its substrate specificity. AcpA-mediated acetate transport is detected from the onset of conidiospore germination, peaks at the time of germ tube emergence, and drops to low basal levels in germlings and young mycelia, where a second acetate transporter is also becoming apparent. AcpA activity also responds to acetate presence in the growth medium, but is not subject to either carbon or nitrogen catabolite repression. Short-chain monocarboxylates (benzoate, formate, butyrate and propionate) inhibit AcpA-mediated acetate transport with apparent inhibition constants (Ki) of 16.89±2.12, 9.25±1.01, 12.06±3.29 and 1.44±0.13mM, respectively. AcpA is also shown not to be directly involved in ammonia export, as proposed for its Saccharomyces cerevisiae homologue Ady2p. In the second part of this work, we search for the unknown acetate transporter expressed in mycelia, and for other transporters that might contribute to acetate uptake. In silico analysis, genetic construction of relevant null mutants, and uptake assays, reveal that the closest AcpA homologue (AN1839), named AcpB, is the 'missing' secondary acetate transporter in mycelia. We also identify two major short-chain carboxylate (lactate, succinate, pyruvate and malate) transporters, named JenA (AN6095) and JenB (AN6703), which however are not involved in acetate uptake. This work establishes a framework for further exploiting acetate and carboxylate transport in filamentous ascomycetes. | por |
dc.description.sponsorship | We are very grateful to Emila Krypotou for her help in the phylogenetic analyses. We are grateful to Dr. Velot for his kind gift of the two strains acpA<SUP>+</SUP> and acpA Delta indispensable for this work. This work was supported by FEDER, through POFC - COMPETE and by Portuguese National Funds from "FCT - Fundacao para a Ciencia e a Tecnologia", in the scope of the projects PEst-C/BIA/UI4050/2011 and PEst-OE/BIA/UI4050/2014. JSP [SFRH/BD/61530/2009] received a fellowship from the Portuguese government from FCT through POPH and FSE. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | PEst-C/BIA/UI4050/2011 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/135919/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F61530%2F2009/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | Acetate | por |
dc.subject | Ammonium Compounds | por |
dc.subject | Aspergillus nidulans | por |
dc.subject | Mycelium | por |
dc.subject | Substrate Specificity | por |
dc.subject | Gene Expression Regulation, Fungal | por |
dc.subject | Membrane Transport | por |
dc.subject | Filamentous fungi | por |
dc.subject | Carbon catabolism | por |
dc.subject | Germination | por |
dc.subject | Specificity | por |
dc.title | Expression and specificity profile of the major acetate transporter AcpA in Aspergillus nidulans | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
oaire.citationStartPage | 93-103 | por |
oaire.citationEndPage | 103 | por |
oaire.citationVolume | 76 | por |
dc.identifier.eissn | 1096-0937 | por |
dc.identifier.doi | 10.1016/j.fgb.2015.02.010 | por |
dc.identifier.pmid | 25708319 | por |
dc.subject.fos | Ciências Naturais::Ciências Biológicas | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Fungal Genetics and Biology | por |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2015-A74. FungalGenetics-AcpA.pdf Acesso restrito! | 2,45 MB | Adobe PDF | Ver/Abrir |