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https://hdl.handle.net/1822/47358
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Campo DC | Valor | Idioma |
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dc.contributor.author | Cunha, Joana T. | por |
dc.contributor.author | Domingues, Lucília | por |
dc.date.accessioned | 2017-11-14T14:34:05Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Cunha, Joana T.; Domingues, Lucília, RAPD/SCAR approaches for identification of adulterant breeds' milk in dairy products. In Lucília Domingues (Ed.), PCR: Methods and Protocols, Vol. Methods in Molecular Biology 1620, New York: Springer, 2017. ISBN: 978-1-4939-7060-5, 183-193 | por |
dc.identifier.isbn | 978-1-4939-7059-9 | por |
dc.identifier.issn | 1064-3745 | por |
dc.identifier.uri | https://hdl.handle.net/1822/47358 | - |
dc.description.abstract | Food safety and quality are nowadays a major consumers concern. In the dairy industry the fraudulent addition of cheaper/lower-quality milks from nonlegitimate species/breeds compromises the quality and value of the final product. Despite the already existing approaches for identification of the species origin of milk, there is little information regarding differentiation at an intra-species level. In this protocol we describe a low-cost, sensitive, fast, and reliable analytical techniqueRandom Amplified Polymorphic DNA/Sequence Characterized Amplified Region (RAPD/SCAR)capable of an efficient detection of adulterant breeds in milk mixtures used for fraudulent manufacturing of dairy products and suitable for the detection of milk adulteration in processed dairy foods. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020— Programa Operacional Regional do Norte, and project “Valor Queijo” (CENTRO-07-0202-FEDER-030372) funded by FCT/ MCTES (PIDDAC) and cofunded by “Fundo Europeu de Desenvolvimento Regional” (FEDER) through “COMPETE— Programa Operacional Factores de Competitividade” (POFC). | por |
dc.language.iso | eng | por |
dc.publisher | Springer | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.rights | closedAccess | por |
dc.subject | DNA extraction | por |
dc.subject | RAPD | por |
dc.subject | SCAR | por |
dc.subject | Breed identification | por |
dc.subject | Milk adulteration | por |
dc.subject | Dairy authentication | por |
dc.subject | Food quality control | por |
dc.title | RAPD/SCAR approaches for identification of adulterant breeds' milk in dairy products | por |
dc.type | bookPart | por |
dc.peerreviewed | yes | - |
dc.relation.publisherversion | https://link.springer.com/protocol/10.1007/978-1-4939-7060-5_16 | por |
dc.comments | CEB46796 | por |
oaire.citationStartPage | 183 | por |
oaire.citationEndPage | 193 | por |
oaire.citationConferencePlace | New York, New York, NY | - |
oaire.citationVolume | Methods in Molecular Biology 1620 | - |
dc.date.updated | 2017-10-29T18:23:08Z | - |
dc.identifier.doi | 10.1007/978-1-4939-7060-5_13 | por |
dc.identifier.eisbn | 978-1-4939-7060-5 | por |
dc.identifier.pmid | 28540709 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Methods in Molecular Biology | por |
sdum.conferencePublication | PCR: METHODS AND PROTOCOLS | por |
sdum.bookTitle | PCR: methods and protocols | por |
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