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https://hdl.handle.net/1822/46851
Título: | Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis |
Autor(es): | Pesqueira, T. Almeida, Raquel Costa Gomes, Manuela E. |
Palavras-chave: | Contact-free therapy Immunomodulation Magnetotherapy Remote actuation Tendon mechanotransduction Tendon-related markers |
Data: | Set-2017 |
Editora: | Nature Publishing Group |
Revista: | Scientific Reports |
Citação: | Pesqueira T., Costa-Almeida R., Gomes M. E. Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis, Scientific Reports, Vol. 7, pp. 10948, doi:10.1038/s41598-017-11253-6, 2017 |
Resumo(s): | Magnetotherapy has been receiving increased attention as an attractive strategy for modulating cell physiology directly at the site of injury, thereby providing the medical community with a safe and non- invasive therapy. Yet, how magnetic eld in uences tendon cells both at the cellular and molecular levels remains unclear. Thus, the in uence of a low-frequency static magnetic eld (2 Hz, 350 mT) on human tendon-derived cells was studied using di erent exposure times (4 and 8 h; short-term studies) and di erent regimens of exposure to an 8h-period of magnetic stimulation (continuous, every 24 h or every 48 h; long-term studies). Herein, 8 h stimulation in short-term studies signi cantly upregulated the expression of tendon-associated genes SCX, COL1A1, TNC and DCN (p < 0.05) and altered intracellular Ca2+ levels (p < 0.05). Additionally, every 24 h regimen of stimulation signi cantly upregulated COL1A1, COL3A1 and TNC at day 14 in comparison to control (p < 0.05), whereas continuous exposure di erentially regulated the release of the immunomodulatory cytokines IL-1β and IL-10 (p < 0.001) but only at day 7 in comparison to controls. Altogether, these results provide new insights on how low-frequency static magnetic eld ne-tune the behaviour of tendon cells according to the magnetic settings used, which we foresee to represent an interesting candidate to guide tendon regeneration. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/46851 |
DOI: | 10.1038/s41598-017-11253-6 |
ISSN: | 2045-2322 |
e-ISSN: | 2045-2322 |
Versão da editora: | https://www.nature.com/articles/s41598-017-11253-6 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19180-Pesqueira et al 2017_Scientific Reports.pdf | 11,78 MB | Adobe PDF | Ver/Abrir |