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https://hdl.handle.net/1822/46766
Título: | Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization |
Autor(es): | Gonçalves, Clarisse Salomé Nobre Alves Filho, E. G. Fernandes, Fabiano A. N. Brito, Edy S. Rodrigues, S. Teixeira, J. A. Rodrigues, L. R. |
Palavras-chave: | Fructo-oligosaccharides Aspergillus ibericus Chemical structure Experimental design Production yield optimization |
Data: | Mar-2018 |
Editora: | Elsevier 1 |
Revista: | LWT - Food Science and Technology |
Citação: | Nobre, Clarisse; Alves Filho, E. G.; Fernandes, Fabiano A. N.; Brito, Edy S.; Rodrigues, S.; Teixeira, José A.; Rodrigues, Lígia R., Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization. LWT - Food Science and Technology, 89, 58-64, 2018 |
Resumo(s): | A great demand for prebiotics is driving the search for new sources of fructo-oligosaccharides (FOS) producers and for FOS with differentiated functionalities. In the present work, FOS production by a new isolated strain of Aspergillus ibericus was evaluated. The temperature of fermentation and initial pH were optimized in shaken flask to yield a maximal FOS production, through a central composite experimental design. FOS were produced in a one-step bioprocess using the whole cells of the microorganism. The model (R2 = 0.918) predicted a yield of 0.56, experimentally 0.53 ± 0.03 gFOS.ginitial sucrose1 was obtained (37.0 °C and a pH of 6.2). A yield of 0.64 ± 0.02 gFOS.ginitial sucrose1 was obtained in the bioreactor, at 38 h, with a content of 118 ± 4 g.L1 in FOS and a purity of 56 ± 3%. The chemical structure of the FOS produced by A. ibericus was determined by HPLC and NMR. FOS were identified as 1-kestose, nystose, and 1F-fructofuranosylnystose. In conclusion, A. ibericus was found to be a good alternative FOS producer. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/46766 |
DOI: | 10.1016/j.lwt.2017.10.015 |
ISSN: | 0023-6438 |
Versão da editora: | http://www.journals.elsevier.com/lwt-food-science-and-technology/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_47026_1.pdf | 538,47 kB | Adobe PDF | Ver/Abrir |