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https://hdl.handle.net/1822/44851
Título: | Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor |
Autor(es): | Pandey, Kishor Ferreira, Pedro Eduardo Mendes Ishikawa, Takeshi Nagai, Takeharu Kaneko, Osamu Yahata, Kazuhide |
Data: | Mar-2016 |
Editora: | Nature Publishing Group |
Revista: | Scientific Reports |
Citação: | Pandey, K., Ferreira, P. E., Ishikawa, T., Nagai, T., Kaneko, O., & Yahata, K. (2016). Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor. Scientific reports, 6. doi: 10.1038/srep23454 |
Resumo(s): | Calcium (Ca2+)-mediated signaling is a conserved mechanism in eukaryotes, including the human malaria parasite, Plasmodium falciparum. Due to its small size (<10?µm) measurement of intracellular Ca2+ in Plasmodium is technically challenging, and thus Ca2+ regulation in this human pathogen is not well understood. Here we analyze Ca2+ homeostasis via a new approach using transgenic P. falciparum expressing the Ca2+ sensor yellow cameleon (YC)-Nano. We found that cytosolic Ca2+ concentration is maintained at low levels only during the intraerythrocytic trophozoite stage (30?nM), and is increased in the other blood stages (>300?nM). We determined that the mammalian SERCA inhibitor thapsigargin and antimalarial dihydroartemisinin did not perturb SERCA activity. The change of the cytosolic Ca2+ level in P. falciparum was additionally detectable by flow cytometry. Thus, we propose that the developed YC-Nano-based system is useful to study Ca2+ signaling in P. falciparum and is applicable for drug screening. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/44851 |
DOI: | 10.1038/srep23454 |
ISSN: | 2045-2322 |
Versão da editora: | http://www.nature.com/articles/srep23454 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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srep23454.pdf | 1,52 MB | Adobe PDF | Ver/Abrir |