Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/44063
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Raju, B. Rama | por |
dc.contributor.author | Gonçalves, M. Sameiro T. | por |
dc.contributor.author | Coutinho, Paulo J. G. | por |
dc.date.accessioned | 2017-01-02T10:07:48Z | - |
dc.date.available | 2017-01-02T10:07:48Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | B. R. Raju, M. S. T. Gonçalves, P. J. G. Coutinho, “Fluorescent probes based on side-chain chlorinated benzo[a]phenoxazinium chlorides: studies of interaction with DNA”, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2017, 171, 1–9. | por |
dc.identifier.issn | 1386-1425 | por |
dc.identifier.uri | https://hdl.handle.net/1822/44063 | - |
dc.description.abstract | The interaction of DNA with six water soluble benzo[a]phenoxazinium chlorides mono- or di-substituted with 3-chloropropyl groups at the O and N of 2- and 9-positions, along with methyl, hydroxyl and amine terminal groups at 5-positions, was investigated by photophysical techniques. The results indicated that almost all compounds intercalated in DNA base pairs at phosphate to dye ratio higher than 5. At lower values of this ratio, electrostatic binding mode with DNA was observed. Groove binding was detected mainly for the benzo[a]phenoxazinium dye with NH2.HBr terminal. The set of six benzo[a]phenoxazinium chlorides proved successful to label the migrating DNA in agarose gel electrophoresis assays. These finding proves the ability of these benzo[a]phenoxazinium dyes to strongly interact with DNA. | por |
dc.description.sponsorship | Thanks are due to Fundação para a Ciência e a Tecnologia (FCT, Portugal) and FEDER (European Fund for Regional Development)/COMPETE-QREN-EU for financial support to Research Centres CQ/UM [PEst-C/QUI/UI0686/2013 (FCOMP-01-0124-FEDER-037302)] and CFUM [PEst-C/FIS/UI0607/2013 (F-COMP-01-0124-FEDER-022711)]. The Postdoctoral grant to B. R. Raju (SFRH/BPD/62881/2009) is also acknowledged to FCT, POPHQREN, FSE. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | PEst-C/QUI/UI0686/2013 (FCOMP-01-0124-FEDER-037302) | por |
dc.relation | PEst-C/FIS/UI0607/2013 (F-COMP-01-0124-FEDER-022711) | por |
dc.relation | SFRH/BPD/62881/2009 | por |
dc.rights | openAccess | por |
dc.subject | Nile Blue | por |
dc.subject | Benzo[a]phenoxazinium chlorides | por |
dc.subject | Fluorescent probes | por |
dc.subject | DNA interaction | por |
dc.subject | Fluorescence anisotropy | por |
dc.subject | Gel electrophoresis | por |
dc.title | Fluorescent probes based on side-chain chlorinated benzo[a]phenoxazinium chlorides: studies of interaction with DNA | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S1386142516304218 | por |
sdum.publicationstatus | info:eu-repo/semantics/acceptedVersion | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 9 | por |
oaire.citationTitle | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | por |
oaire.citationVolume | 171 | por |
dc.identifier.doi | 10.1016/j.saa.2016.07.030 | por |
dc.identifier.pmid | 27450866 | por |
dc.subject.fos | Ciências Naturais::Ciências Químicas | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy | por |
Aparece nas coleções: | CDQuim - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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MSTG-Final revised manuscript-2017.pdf | 2,54 MB | Adobe PDF | Ver/Abrir |