Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/43825
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Martins, Margarida Isabel Barros Coelho | por |
dc.contributor.author | Ribeiro, Diana Margarida da Costa | por |
dc.contributor.author | Martins, Albino | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Neves, N. M. | por |
dc.date.accessioned | 2016-12-23T10:23:36Z | - |
dc.date.available | 2016-12-23T10:23:36Z | - |
dc.date.issued | 2016-03 | - |
dc.date.submitted | 2016-03 | - |
dc.identifier.citation | Martins M., Ribeiro D., Martins A., Reis R. L., Neves N. M. Extracellular Vesicles Derived from Osteogenically Induced Human Bone Marrow Mesenchymal Stem Cells Can Modulate Lineage Commitment, Stem Cell Reports , Vol. 6, Issue 3, pp. 284-291, doi:10.1016/j.stemcr.2016.01.001, 2016 | por |
dc.identifier.issn | 2213-6711 | por |
dc.identifier.uri | https://hdl.handle.net/1822/43825 | - |
dc.description.abstract | The effective osteogenic commitment of human bone marrow mesenchymal stem cells (hBMSCs) is critical for bone regenerative therapies. Extracellular vesicles (EVs) derived from hBMSCs have a regenerative potential that has been increasingly recognized. Herein, the osteoinductive potential of osteogenically induced hBMSC-EVs was examined. hBMSCs secreted negatively charged nanosized vesicles (⠼35 nm) with EV-related surface markers. The yield of EVs over 7 days was dependent on an osteogenic stimulus (standard chemical cocktail or RUNX2 cationic-lipid transfection). These EVs were used to sequentially stimulate homotypic uncommitted cells during 7 days, matching the seeding density of EV parent cells, culture time, and stimuli. Osteogenically committed hBMSC-EVs induced an osteogenic phenotype characterized by marked early induction of BMP2, SP7, SPP1, BGLAP/IBSP, and alkaline phosphatase. Both EV groups outperformed the currently used osteoinductive strategies. These data show that naturally secreted EVs can guide the osteogenic commitment of hBMSCs in the absence of other chemical or genetic osteoinductors. | por |
dc.description.sponsorship | The authors thank the financial support of QREN (RL1-ABMRNORTE-01-0124-FEDER-000016 and RL3–TECT–NORTE-01-0124- FEDER-000020) co-financed by North Portugal Regional Operational Program (ON.2–O Novo Norte), under the NSRF, through the ERDF; the European Union’s Seventh Framework Program (FP7/2007–2013), Grant No. REGPOT-CT2012-316331- POLARIS; and the laboratory assistance of Elsa Ribeiro, Magda Graça, and Sónia Zacarias. | por |
dc.language.iso | eng | por |
dc.publisher | Cell Press | por |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/316331/EU | - |
dc.rights | openAccess | por |
dc.subject | Advanced therapies/ bone regeneration | por |
dc.subject | Differentiation | por |
dc.subject | Exosome | por |
dc.subject | Osteogenic commitment strategies | por |
dc.subject | Secretome | por |
dc.subject | Stem cells | por |
dc.title | Extracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.cell.com/stem-cell-reports/pdf/S2213-6711(16)00002-3.pdf | por |
dc.comments | http://3bs.uminho.pt/node/18674 | por |
sdum.publicationstatus | info:eu-repo/semantics/publishedVersion | por |
oaire.citationStartPage | 284 | por |
oaire.citationEndPage | 291 | por |
oaire.citationIssue | 3 | por |
oaire.citationTitle | Stem Cell Reports | por |
oaire.citationVolume | 6 | por |
dc.date.updated | 2016-12-14T15:34:50Z | - |
dc.identifier.doi | 10.1016/j.stemcr.2016.01.001 | por |
dc.identifier.pmid | 26923821 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Stem Cell Reports | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18674-EVS.pdf | 1,49 MB | Adobe PDF | Ver/Abrir |