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https://hdl.handle.net/1822/43069
Título: | Fluorescent quantification of melanin |
Autor(es): | Fernandes, Bruno Pacheco Matamá, Maria Teresa Guimarães, Diana Isabel Pereira Gomes, Andreia C Cavaco-Paulo, Artur |
Palavras-chave: | Melanin Pigment quantification Melanin oxidation Melanin fluorescence Absorption spectroscopy |
Data: | 2016 |
Editora: | John Wiley and Sons |
Revista: | Pigment Cell and Melanoma Research |
Citação: | Fernandes, Bruno P.; Matamá, Maria Teresa; Guimarães, D.; Gomes, Andreia; Cavaco-Paulo, Artur, Fluorescent quantification of melanin. Pigment Cell and Melanoma Research, 29(6), 707-712, 2016 |
Resumo(s): | Melanin quantification is reportedly performed by absorption spectroscopy, commonly at 405 nm. Here, we propose the implementation of fluorescence spectroscopy for melanin assessment. In a typical in vitro assay to assess melanin production in response to an external stimulus, absorption spectroscopy clearly overvalues melanin content. This method is also incapable of distinguishing non-melanotic/amelanotic control cells from those that are actually capable of performing melanogenesis. Therefore, fluorescence spectroscopy is the best method for melanin quantification as it proved to be highly specific and accurate, detecting even small variations in the synthesis of melanin. This method can also be applied to the quantification of melanin in more complex biological matrices like zebrafish embryos and human hair. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/43069 |
DOI: | 10.1111/pcmr.12535 |
ISSN: | 1755-1471 |
e-ISSN: | 1755-148X |
Versão da editora: | http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1755-148X |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series CBMA - Artigos/Papers DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_46218_1.pdf | 653,89 kB | Adobe PDF | Ver/Abrir |