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https://hdl.handle.net/1822/42334
Título: | Simultaneous determination of Deoxynivalenol, Deoxynivalenol-3-Glucoside and Nivalenol in wheat grains by HPLC-PDA with immunoaffinity column cleanup |
Autor(es): | Trombete, F. Barros, A. Vieira, Madalena Saldanha, Tatiana Venâncio, Armando Fraga, M. |
Palavras-chave: | Modified mycotoxin Trichothecene D3G DON Method validation |
Data: | Set-2016 |
Editora: | Springer Verlag |
Revista: | Food Analytical Methods |
Citação: | Trombete, F.; Barros, A.; Vieira, Madalena; Saldanha, Tatiana; Venâncio, Armando; Fraga, M., Simultaneous Determination of Deoxynivalenol, Deoxynivalenol-3-Glucoside and Nivalenol in Wheat Grains by HPLC-PDA with Immunoaffinity Column Cleanup. Food Analytical Methods, 9(9), 2579-2586, 2016 |
Resumo(s): | Deoxynivalenol-3-glucoside (D3G) is a modified mycotoxin formed by the metabolism of plants through the conjugation of deoxynivalenol (DON) with glucose. Toxicology studies of D3G for human and animal health are still under investigation, and the development of practical and reliable methods for its direct determination, especially in cereal matrices, is of great importance. In the present study, a methodology for simultaneous determination of D3G, DON, and nivalenol (NIV) in wheat grains, using immunoaffinity column (IAC) cleanup, separation by C18 column and detection by ultraviolet (UV) absorption, was optimized and in-house validated. The results demonstrated adequate values of D3G recovery from IAC and spiked samples. Intraday precision, linearity, limit of detection and limit of quantification (LOQ) were also adequate for the determination of these mycotoxins. Range of applicability varied from 47.1 to 1000 g/kg for D3G and from 31.3 to 1000 g/kg for DON and NIV, with recovery ranging from 84.7±7.2 % to 112.3±8.1 |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/42334 |
DOI: | 10.1007/s12161-016-0450-5 |
ISSN: | 1936-9751 |
e-ISSN: | 1936-976X |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_37815_1.pdf | 497,07 kB | Adobe PDF | Ver/Abrir |