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https://hdl.handle.net/1822/41538
Título: | Compositional analysis of biofilms formed by Staphylococcus aureus isolated from food sources |
Autor(es): | Oniciuc, Elena-Alexandra Cerca, Nuno Nicolau, Anca I. |
Palavras-chave: | Staphylococcus aureus biofilm food CLSM exopolysaccharide protein biofilm |
Data: | 30-Mar-2016 |
Editora: | Frontiers Media |
Revista: | Frontiers in Microbiology |
Citação: | Oniciuc, Elena-Alexandra; Cerca N; Nicolau, Anca I., Compositional analysis of biofilms formed by Staphylococcus aureus isolated from food sources. Frontiers in Microbiology, 7(370), 1-4, 2016 |
Resumo(s): | Sixteen Staphylococcus aureus isolatesoriginatingfromfoods(eightfromdairyproducts, five from fish and fish products and three from meat and meat products) were evaluated regarding their biofilms formation ability. Six strains (E2, E6, E8, E10, E16, and E23) distinguished as strong biofilm formers, either in standard Tryptic Soy Broth or in Tryptic Soy Broth supplemented with 0.4% glucose or with 4% NaCl. The composition of the biofilms formed by these S. aureus strains on polystyrene surfaces was first inferred using enzymatic and chemical treatments. Later on, biofilms were characterized by confocal laser scanning microscope (CLSM). Our experiments proved that protein-based matrices are of prime importance for the structure of biofilms formed by S. aureus strains isolated from food sources. These biofilm matrix compositions are similar to those put into evidence for coagulase negative staphylococci. This is a new finding having in view that scientific literature mentions exopolysaccharide abundance in biofilms produced by clinical isolates and food processing environment isolates of S. aureus. |
Tipo: | Artigo |
Descrição: | The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fmicb. 2016.00390 |
URI: | https://hdl.handle.net/1822/41538 |
DOI: | 10.3389/fmicb.7016.00390 |
ISSN: | 1664-302X |
e-ISSN: | 1664-302X |
Versão da editora: | http://journal.frontiersin.org/journal/microbiology |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_38947_1.pdf | 796,08 kB | Adobe PDF | Ver/Abrir |