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https://hdl.handle.net/1822/40669
Título: | Development of Janus hydrogel microcapsules as novel biomaterials-stem cells construct for 3D co-culture applications |
Autor(es): | Bishi, D. K. Gasperini, L. Marques, A. P. Mano, J. F. Reis, R. L. |
Palavras-chave: | Co-cultures hydrogel Microencapsulation Microfluidics |
Data: | Ago-2015 |
Editora: | Mary Ann Liebert |
Revista: | Tissue Engineering. Part A |
Citação: | Bishi, D. K., Gasperini, L., Marques, A. P., Mano, J. F., & Reis, R. L. (2015). Development of Janus Hydrogel Microcapsules as Novel Biomaterials-Stem Cells Construct for 3D Co-culture Applications. Tissue Engineering Part A, 21, S255-S255. |
Resumo(s): | Co-cultures of two or more cell types and biodegradable biomaterials of natural origin have been successfully combined to recreate tissue microenvironments. Segregated co-cultures are preferred over conventional mixed ones in order to better control the degree of homotypic and heterotypic interactions. Hydrogel-based systems in particular, have gained much attention to mimic tissue-specific microenvironments and they can be microengineered by innovative bottom-up approaches such as microfluidics. In this study, we developed bi-compartmentalized (Janus) hydrogel microcapsules of methacrylated hyaluronic acid (MeHA)/methacrylated-chitosan (MeCht) blended with marine-origin collagen by droplet-based microfluidics co-flow. Human adipose stem cells (hASCs) and microvascular endothelial cells (hMVECs) were co-encapsulated to create platforms of study relevant for vascularized bone tissue engineering. A specially designed Janus-droplet generator chip was used to fabricate the microcapsules (<250â μm units) and Janus-gradient co-cultures of hASCs: hMVECs were generated in various ratios (90:10; 75:25; 50:50; 25:75; 10:90), through an automated microfluidic flow controller (Elveflow microfluidics system). Such monodisperse 3D co-culture systems were optimized regarding cell number and culture media specific for concomitant maintenance of both phenotypes to establish effective cell-cell (homotypic and heterotypic) and cell-materials interactions. Cellular parameters such as viability, matrix deposition, mineralization and hMVECs re-organization in tube-like structures, were enhanced by blending MeHA/MeCht with marine-origin collagen and increasing hASCs: hMVECs co-culture gradient had significant impact on it. Such Janus hybrid hydrogel microcapsules can be used as a platform to investigate biomaterials interactions with distinct combined cell populations. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/40669 |
ISSN: | 2152-4947 |
Versão da editora: | http://online.liebertpub.com/doi/10.1089/ten.tea.2015.5000.abstracts |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18629-dillip.pdf | 27,57 kB | Adobe PDF | Ver/Abrir |