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https://hdl.handle.net/1822/37874
Título: | Factorial design for collagenase production by Penicillium sp. selected from the Caatinga soil |
Autor(es): | Wanderley, Maria Carolina de Albuquerque Lima, Carolina Albuquerque Silvério, Sara C. Teixeira, J. A. Porto, Ana Lúcia Figueiredo |
Data: | Set-2015 |
Citação: | Wanderley, M.; Lima, Carolina Albuquerque; Silvério, S.; Teixeira, J. A.; Porto, Ana Lúcia Figueiredo, Factorial design for collagenase production by Penicillium sp. selected from the Caatinga soil. SINAFERM 2015 XX Simpósio Nacional de Bioprocessos. Fortaleza, Brazil, Sep. 1-4, 1-6, 2015. |
Resumo(s): | Collagenolytic proteases can hydrolyze both denatured and native collagen and are becoming increasingly important commercially. The aim of this work was to select a new strain of Penicillium sp. isolated from the soil of Caatinga for collagenase production. A factorial design 24 was performed to determine the best conditions of enzyme production. Collagenolytic activity reported on this work is about 2 times larger than existing data. According to the growth kinetics, after 126 hours of production, were obtained the highest values of collagenolytic activity and specific activity. The highest values of collagenolytic activity and specific activity were obtained on a culture medium containing 0.25% (w/v) gelatin, 200 rpm, pH 8.0 and 24 °C. Only two factors were statistically significant: pH and temperature, both with negative effects. The experimental design made possible to define fermentation culture conditions able to increase by 66% the value of the initial enzyme activity. |
Tipo: | Artigo em ata de conferência |
URI: | https://hdl.handle.net/1822/37874 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Artigos em Livros de Atas / Papers in Proceedings |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_23425_1.pdf | 170,88 kB | Adobe PDF | Ver/Abrir |