Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/37622
Título: | Peptide anchor for folate-targeted liposomal delivery |
Autor(es): | Nogueira, E. Mangialavori, Irene C. Loureiro, Ana Azóia, Nuno G. Sarria, Marisa P. Nogueira, Patrícia Freitas, Jaime Harmark, Johan Shimanovich, Ulyana Rollett, Alexandra Lacroix, Ghislaine Bernardes, Gonçalo J. L. Guebitz, Georg Hebert, Hans Moreira, Alexandra Carmo, Alexandre M. Rossi, Juan Pablo F. C. Gomes, Andreia C Preto, Ana Cavaco-Paulo, Artur |
Data: | 4-Ago-2015 |
Editora: | ACS Publications |
Revista: | Biomacromolecules |
Citação: | Nogueira, E.; Irene C. Mangialavori; Loureiro, A.; Azoia, Nuno G.; Passos, M.; Patrícia Nogueira; Jaime Freitas; Harmark, J.; Ulyana Shimanovich; Ulyana Shimanovich; Alexandra Rollett; Ghislaine Lacroix; Bernardes, Gonçalo J. L.; Guebitz, Georg; Hebert, H.; Alexandra Moreira; Alexandre M. Carmo; Juan Pablo F. C. Rossi; Andreia Gomes; Ana Preto; Paulo, Artur Cavaco, Peptide anchor for folate-targeted liposomal delivery. Biomacromolecules, 16(9), 2904-2910, 2015 |
Resumo(s): | Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fragment of surfactant protein D linked to folate. Our proposed spacer is a small 4 amino acid residue linker. The peptide conjugate inserts deeply into the lipid bilayer without affecting liposomal integrity, with high stability and specificity. To compare the drug delivery potential of both liposomal targeting systems, we encapsulated the nuclear dye Hoechst 34580. The eventual increase in blue fluorescence would only be detectable upon liposome disruption, leading to specific binding of this dye to DNA. Our delivery system was proven to be more efficient (2-fold) in Caco-2 cells than classic systems where the folate moiety is linked to liposomes by polyethylene glycol. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/37622 |
ISBN: | 1526-4602 |
DOI: | 10.1021/acs.biomac.5b00823 |
ISSN: | 1525-7797 |
e-ISSN: | 1526-4602 |
Versão da editora: | http://pubs.acs.org/journal/bomaf6 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series CBMA - Artigos/Papers DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_22388_1.pdf | 2,38 MB | Adobe PDF | Ver/Abrir |