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https://hdl.handle.net/1822/37595
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Campo DC | Valor | Idioma |
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dc.contributor.author | Nobre, Clarisse | por |
dc.contributor.author | Castro, C. C. | por |
dc.contributor.author | Hantson, A. -L. | por |
dc.contributor.author | Teixeira, J. A. | por |
dc.contributor.author | Weireld, G. De | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.date.accessioned | 2015-10-09T10:47:25Z | - |
dc.date.available | 2015-10-09T10:47:25Z | - |
dc.date.issued | 2016-01-20 | - |
dc.identifier.citation | Nobre, C.; Castro, C. C.; Hantson, A. -L.; Teixeira, J. A.; Weireld, G. De ; Rodrigues, L. R., Strategies for the production of high-content fructo-oligosaccharides through the removal of small saccharides by co-culture or successive fermentation with yeast. Carbohydrate Polymers, 136, 274-281, 2016 | por |
dc.identifier.issn | 0144-8617 | por |
dc.identifier.uri | https://hdl.handle.net/1822/37595 | - |
dc.description.abstract | Fructo-oligosaccharides (FOS) obtained by fermentation of sucrose may be purified at large-scale by continuous chromatography (Simulated Moving Bed: SMB). In order to improve the efficiency of the subsequent SMB purification, the optimization of the fermentative broth composition in salts and sugars was investigated. Fermentations conducted at reduced amount of salts, using Aureobasidium pullulans whole cells, yielded 0.63 ± 0.03 g of FOS per gram of initial sucrose. Additionally, a microbial treatment was proposed to reduce the amount of small saccharides in the mixture. Two approaches were evaluated, namely a co-culture of A. pullulans with Saccharomyces cerevisiae; and a two-step fermentation in which FOS were first synthesized by A. pullulans and then the small saccharides were metabolised by S. cerevisiae. Assays were performed in 100 mL shaken flasks and further scaled-up to a 3 L working volume bioreactor. Fermentations in two-step were found to be more efficient than the co-culture ones. FOS were obtained with a purity of 81.6 ± 0.8% (w/w), on a dry weight basis, after the second-step fermentation with S. cerevisiae. The sucrose amount was reduced from 13.5 to 5.4% in total sugars, which suggests that FOS from this culture broth will be more efficiently separated by SMB. | por |
dc.description.sponsorship | The financial support from the F.R.S.-FNRS, the Belgium National Fund for the Scientific Research, (PDR: T.0196.13) is gratefully acknowledged. Clarisse Nobre acknowledges to Fundacao para a Ciencia e a Tecnologia (Portugal) and POPH/FSE for the Post-Doc Grant received (reference SFRH/BPD/87498/2012). The authors acknowledge the financial support from the Strategic funding of UID/BIO/04469/2013 unit and project ref. RECI/BBB-EBI/0179/2012 (project number FCOMP-01-0124-FEDER-027462) funded by Fundacao para a Ciencia e a Tecnologia. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | openAccess | por |
dc.subject | Fructo-oligosaccharides | por |
dc.subject | Aureobasidium pullulans | por |
dc.subject | Co-culture | por |
dc.subject | Successive Fermentation | por |
dc.subject | Saccharomyces cerevisiae | por |
dc.title | Strategies for the production of high-content fructo-oligosaccharides through the removal of small saccharides by co-culture or successive fermentation with yeast | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/carbohydrate-polymers/ | por |
dc.comments | CEB22356 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 274 | por |
oaire.citationEndPage | 281 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationTitle | Carbohydrate Polymers | por |
oaire.citationVolume | 136 | por |
dc.date.updated | 2015-09-26T20:59:04Z | - |
dc.identifier.eissn | 0144-8617 | - |
dc.identifier.doi | 10.1016/j.carbpol.2015.08.088 | por |
dc.identifier.pmid | 26572356 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Carbohydrate Polymers | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_22356_1.pdf | 780,18 kB | Adobe PDF | Ver/Abrir |