Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/37132
Título: | IgG and fibrinogen driven nanoparticle aggregation |
Autor(es): | Cukalevski, Risto Ferreira, Sílvia A. Dunning, Christopher J. Berggård, Tord Cedervall, Tommy |
Palavras-chave: | Nanoparticles (NPs) Protein Corona Aggregation Immunoglobulin |
Data: | 2015 |
Editora: | Tsinghua University Press |
Revista: | Nano Research |
Resumo(s): | A thorough understanding of how proteins induce nanoparticle (NP) aggregation is crucial when designing in vitro and in vivo assays and interpreting experimental results. This knowledge is also crucial when developing nano-applications and formulation for drug delivery systems. In this study, we found that extraction of immunoglobulin G (IgG) from cow serum results in lower polystyrene NPs aggregation. Moreover, addition of isolated IgG or fibrinogen to fetal cow serum enhanced this aggregation, thus demonstrating that these factors are major drivers of NP aggregation in serum. Counter-intuitively, NP aggregation was inversely dependent on protein concentration; i.e., low protein concentrations induced large aggregates, whereas high protein concentrations induced small aggregates. Protein-induced NP aggregation and aggregate size were monitored by absorbance at 400 nm and dynamic light scattering, respectively. Here, we propose a mechanism behind the protein concentration dependent aggregation; this mechanism involves the effects of multiple protein interactions on the NP surface, surface area limitations, aggregation kinetics, and the influence of other serum proteins. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/37132 |
DOI: | 10.1007/s12274-015-0780-4 |
ISSN: | 1998-0124 |
e-ISSN: | 1998-0000 |
Versão da editora: | http://www.springer.com/materials/nanotechnology/journal/12274 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_22210_1.pdf | 1,99 MB | Adobe PDF | Ver/Abrir |