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https://hdl.handle.net/1822/35343
Título: | Analytical protocols for separation and electron microscopy of nanoparticles interacting with bacterial cells |
Autor(es): | Sousa, Cláudia Sequeira, Diana Kolen´ko, Yury V. Pinto, Inês M. Petrovykh, D. Y. |
Data: | Mar-2015 |
Editora: | American Chemical Society |
Revista: | Analytical Chemistry |
Citação: | Sousa, Cláudia; Sequeira, Diana; Kolen'ko, Yury V.; Pinto, Inês M.; Petrovykh, D. Y., Analytical protocols for separation and electron microscopy of nanoparticles interacting with bacterial cells. Analytical Chemistry, 87(9), 4641-4648, 2015 |
Resumo(s): | An important step toward understanding interactions between nanoparticles (NPs) and bacteria is the ability to directly observe NPs interacting with bacterial cells. NPbacteria mixtures typical in nanomedicine, however, are not yet amendable for direct imaging in solution. Instead, evidence of NPcell interactions must be preserved in derivative (usually dried) samples to be subsequently revealed in high-resolution images, e.g., via scanning electron microscopy (SEM). Here, this concept is realized for a mixed suspension of model NPs and Staphylococcus aureus bacteria. First, protocols for analyzing the relative colloidal stabilities of NPs and bacteria are developed and validated based on systematic centrifugation and comparison of colony forming unit (CFU) counting and optical density (OD) measurements. Rate-dependence of centrifugation efficiency for each component suggests differential sedimentation at a specific predicted rate as an effective method for removing free NPs after co-incubation; the remaining fraction comprises bacteria with any associated NPs and can be examined, e.g., by SEM, for evidence of NPbacteria interactions. These analytical protocols, validated by systematic control experiments and high-resolution SEM imaging, should be generally applicable for investigating NPbacteria interactions. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/35343 |
DOI: | 10.1021/ac503835a |
ISSN: | 0003-2700 1520-6882 |
e-ISSN: | 1520-6882 |
Versão da editora: | http://pubs.acs.org/doi/abs/10.1021/ac503835a |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_19854_1.pdf | 2,08 MB | Adobe PDF | Ver/Abrir |