Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/34724
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Campo DC | Valor | Idioma |
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dc.contributor.author | Rodrigues, Joana Lúcia Lima Correia | por |
dc.contributor.author | Araújo, R. G. | por |
dc.contributor.author | Prather, Kristala L. J. | por |
dc.contributor.author | Kluskens, Leon | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.date.accessioned | 2015-04-07T11:08:31Z | - |
dc.date.available | 2015-04-07T11:08:31Z | - |
dc.date.issued | 2015-01-02 | - |
dc.identifier.issn | 1860-6768 | por |
dc.identifier.uri | https://hdl.handle.net/1822/34724 | - |
dc.description.abstract | Curcuminoids are phenylpropanoids with high pharmaceutical potential. Herein, we report an engineered artificial pathway in Escherichia coli to produce natural curcuminoids through caffeic acid. Arabidopsis thaliana 4-coumaroyl-CoA ligase (4CL1) and Curcuma longa diketide-CoA synthase (DCS) and curcumin synthase (CURS1) were used to produce curcuminoids and 70 mg/L of curcumin was obtained from ferulic acid. Bisdemethoxycurcumin and demethoxycurcumin were also produced, but in lower concentrations, by feeding p-coumaric acid or a mixture of p-coumaric acid and ferulic acid, respectively. Additionally, curcuminoids were produced from tyrosine through the caffeic acid pathway. To produce caffeic acid, tyrosine ammonia lyase (TAL) from Rhodotorula glutinis and 4-coumarate 3-hydroxylase (C3H) from Saccharothrix espanaensis were used. Caffeoyl-CoA 3-O-methyltransferase (CCoAOMT) from Medicago sativa was used to convert caffeoyl-CoA to feruloyl-CoA. Using caffeic acid, p-coumaric acid or tyrosine as a substrate, 3.9 mg/L, 0.3 mg/L and 0.2 mg/L of curcumin were produced, respectively. This is the first time DCS and CURS1 were used in vivo to produce curcuminoids and that curcumin was produced by feeding tyrosine. We have shown that curcumin can be produced using a pathway through caffeic acid. This alternative pathway represents a step forward in the heterologous production of curcumin using E. coli. | por |
dc.description.sponsorship | This work was supported by FEDER funds through the COMPETE and ON2 program and through National funds of FCT in the scope of the project FCOMP-01-0124-FEDER-027462, PEst-OE/EQB/LA0023/2013, NORTE-07-0124-FEDER-000028, and NORTE-07-0124-FEDER-000027. Financial support for this work was provided by FCT grant SFRH/BD/51187/2010 and SYNBIOBACTHER project (PTDC/EBB-BIO/102863/2008). | por |
dc.language.iso | eng | por |
dc.publisher | Wiley | por |
dc.rights | openAccess | por |
dc.subject | Caffeic acid | por |
dc.subject | Curcumin synthase | por |
dc.subject | Curcuminoids | por |
dc.subject | E. coli | por |
dc.subject | Tyrosine | por |
dc.subject | E | por |
dc.subject | coli | por |
dc.title | Production of curcuminoids from tyrosine by a metabolically engineered Escherichia coli using caffeic acid as an intermediate | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7314 | por |
dc.comments | CEB19707 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 599 | por |
oaire.citationEndPage | 609 | por |
oaire.citationIssue | 4 | por |
oaire.citationConferencePlace | Germany | - |
oaire.citationTitle | Biotechnology Journal | por |
oaire.citationVolume | 10 | por |
dc.date.updated | 2015-04-03T22:54:27Z | - |
dc.identifier.eissn | 1860-7314 | - |
dc.identifier.doi | 10.1002/biot.201400637 | por |
dc.identifier.pmid | 25641677 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biotechnology Journal | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_19707_1.pdf | 775,32 kB | Adobe PDF | Ver/Abrir |