Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/33281
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Silva, Germana Michelle de Medeiros e | por |
dc.contributor.author | Bezerra, Raquel P. | por |
dc.contributor.author | Teixeira, J. A. | por |
dc.contributor.author | Porto, Tatiana Souza | por |
dc.contributor.author | Lima-Filho, José. L. | por |
dc.contributor.author | Porto, Ana L. F. | por |
dc.date.accessioned | 2015-01-27T19:32:06Z | - |
dc.date.available | 2015-01-27T19:32:06Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Silva, Germana M. M.; Bezerra, Raquel P.; Teixeira, J. A.; Porto, Tatiana S.; Lima-Filho, José L.; Porto, Ana Lúcia F., Fibrinolytic protease production by new Streptomyces sp. DPUA 1576 from Amazon lichens. Electronic Journal of Biotechnology, 18(1), 16-19, 2015 | por |
dc.identifier.issn | 0717-3458 | por |
dc.identifier.uri | https://hdl.handle.net/1822/33281 | - |
dc.description.abstract | Background Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 22 factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentration. A polynomial model was fitted to correlate the relationship between the two variables and protease activity. In relation to fibrinolytic activity, the highest activity of 706.5 mm2 was obtained at 1.7% soybean flour and 1.0% glucose concentration, which was 33% higher than plasmin. Fibrinolytic production was not optimized in the studied conditions. Conclusions These results show that the optimization of the culture medium can enhance protease production, thus becoming a good process for further research. In addition, Streptomyces sp. DPUA 1576, isolated from Amazon lichens, might be a potential strain for fibrinolytic protease production. | por |
dc.description.sponsorship | The authors thank CAPES (National Council for the Improvement of Higher Education) for the scholarship and CNPq/RENORBIO (National Counsel of Technological and Scientific Development, N. 55146/2010-3) and FACEPE (Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco, 0158-2.12/11) for the financial support. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier B.V. | por |
dc.rights | openAccess | por |
dc.subject | Actinomycetes | por |
dc.subject | Factorial design | por |
dc.subject | Glucose | por |
dc.subject | Microbial enzymes | por |
dc.subject | Soybean flour | por |
dc.title | Fibrinolytic protease production by new Streptomyces sp. DPUA 1576 from amazon lichens | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.elsevier.com/locate/issn/07173458 | por |
dc.comments | CEB19059 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 16 | por |
oaire.citationEndPage | 19 | por |
oaire.citationIssue | 1 | por |
oaire.citationConferencePlace | Chile | - |
oaire.citationTitle | Electronic Journal of Biotechnology | por |
oaire.citationVolume | 18 | por |
dc.date.updated | 2015-01-27T18:58:52Z | - |
dc.identifier.doi | 10.1016/j.ejbt.2014.11.001 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Electronic Journal of Biotechnology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_19059_1.pdf | 380,58 kB | Adobe PDF | Ver/Abrir |