Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/31856
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Werner, Johannes | por |
dc.contributor.author | Ferrer, Manuel | por |
dc.contributor.author | Michel, Gurvan | por |
dc.contributor.author | Mann, Alexander J. | por |
dc.contributor.author | Sixing Huang | por |
dc.contributor.author | Juarez, Silvia | por |
dc.contributor.author | Ciordia, Sergio | por |
dc.contributor.author | Albar, Juan P. | por |
dc.contributor.author | Alcaide, María | por |
dc.contributor.author | La Cono, Violetta | por |
dc.contributor.author | Yakimov, Michail M. | por |
dc.contributor.author | Antunes, André Guimarães Lemos | por |
dc.contributor.author | Taborda, Marco | por |
dc.contributor.author | Costa, Milton S. da | por |
dc.contributor.author | Tran Hai | por |
dc.contributor.author | Glöckner, Frank Oliver | por |
dc.contributor.author | Golyshina, Olga V. | por |
dc.contributor.author | Golyshin, Peter N. | por |
dc.contributor.author | Teeling, Hanno | por |
dc.contributor.author | The MAMBA Consortium | por |
dc.date.accessioned | 2014-12-11T14:42:05Z | - |
dc.date.available | 2014-12-11T14:42:05Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Werner, Johannes; Ferrer, Manuel; Michel, Gurvan; Mann, Alexander J.; Huang, Sixing; Juarez, Silvia; Ciordia, Sergio; Albar, Juan P.; Alcaide, María; La Cono, Violetta; Yakimov, Michail M.; Antunes, A.; Taborda, Marco; da Costa, Milton S.; Hai, Tran; Glöckner, Frank Oliver; Golyshina, Olga V.; Golyshin, Peter N.; Teeling, Hanno; The MAMBA Consortium, Halorhabdus tiamatea: proteogenomics and glycosidase activity measurements identify the first cultivated euryarchaeon from a deep-sea anoxic brine lake as potential polysaccharide degrader. Environmental Microbiology, 16(8), 2525-2537, 2014 | por |
dc.identifier.issn | 1462-2912 | por |
dc.identifier.uri | https://hdl.handle.net/1822/31856 | - |
dc.description.abstract | Euryarchaea from the genus Halorhabdus have been found in hypersaline habitats worldwide, yet are represented by only two isolates: Halorhabdus utahensis AX-2T from the shallow Great Salt Lake of Utah, and Halorhabdus tiamatea SARL4BT from the Shaban deep-sea hypersaline anoxic lake (DHAL) in the Red Sea. We sequenced the H. tiamatea genome to elucidate its niche adaptations. Among sequenced archaea, H. tiamatea features the highest number of glycoside hydrolases, the majority of which were expressed in proteome experiments. Annotations and glycosidase activity measurements suggested an adaptation towards recalcitrant algal and plant-derived hemicelluloses. Glycosidase activities were higher at 2% than at 0% or 5% oxygen, supporting a preference for low-oxygen conditions. Likewise, proteomics indicated quinone-mediated electron transport at 2% oxygen, but a notable stress response at 5% oxygen. Halorhabdus tiamatea furthermore encodes proteins characteristic for thermophiles and light-dependent enzymes (e.g. bacteriorhodopsin), suggesting that H. tiamatea evolution was mostly not governed by a cold, dark, anoxic deep-sea habitat. Using enrichment and metagenomics, we could demonstrate presence of similar glycoside hydrolase-rich Halorhabdus members in the Mediterranean DHAL Medee, which supports that Halorhabdus species can occupy a distinct niche as polysaccharide degraders in hypersaline environments. | por |
dc.description.sponsorship | We thank the crew of the R/V Urania for sampling of the Medee DHAL, Jorg Wulf for catalysed reporter deposition fluorescence in situ hybridization analyses, Rafael Bargiela for helping with figures and Rudolf Amann for critical reading of the manuscript. This study was supported by the EU FP7 project MAMBA('Marine Metagenomics for New Biotechnological Applications', FP7-KBBE-2008-226977; http://mamba.bangor.ac.uk/), the Spanish Ministry of Economy and Competitiveness (grant BIO2011-25012) and the Max Planck Society. H. T., O.V.G. and P.N.G. acknowledge the support of EU FP7 for the project MicroB3 (OCEAN-2011-287589). | por |
dc.language.iso | eng | por |
dc.publisher | Blackwell Publishing Inc. | por |
dc.rights | openAccess | por |
dc.title | Halorhabdus tiamatea : proteogenomics and glycosidase activity measurements identify the first cultivated euryarchaeon from a deep-sea anoxic brine lake as potential polysaccharide degrader | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.comments | CEB17984 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 2525 | por |
oaire.citationEndPage | 2537 | por |
oaire.citationIssue | 8 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationTitle | Applied and Environmental Microbiology | por |
oaire.citationVolume | 16 | por |
dc.date.updated | 2014-11-28T17:48:15Z | - |
dc.identifier.eissn | 1462-2920 | - |
dc.identifier.doi | 10.1111/1462-2920.12393 | por |
dc.identifier.pmid | 24428220 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Applied and Environmental Microbiology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_17984_1.pdf | 657,63 kB | Adobe PDF | Ver/Abrir |