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https://hdl.handle.net/1822/31632
Título: | Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology |
Autor(es): | Braga, Adelaide Mesquita, D. P. Amaral, A. L. Ferreira, Eugénio C. Belo, Isabel |
Palavras-chave: | Airlift bioreactor Y. lipolytica gamma-decalactone Oxygen mass transfer rate Cellular morphology Image analysis ?-Decalactone γ-Decalactone |
Data: | 2015 |
Editora: | Elsevier B.V. |
Revista: | Biochemical Engineering Journal |
Citação: | Braga, Adelaide; Mesquita, D. P.; Amaral, A. L.; Ferreira, E. C.; Belo, Isabel, Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology. Biochemical Engineering Journal, 93, 55-62, 2015 |
Resumo(s): | The production of γ-decalactone from castor oil in batch cultures of Yarrowia lipolytica W29 was compared in stirred tank (STR) and airlift bioreactors. Oxygen mass transfer from air to biphasic medium was characterized in both bioreactors trough correlations for kLa with power input and superficial gas velocity. Different conditions of oxygen transfer rate (OTR) were selected to perform biotransformations and for both bioreactors improvement of γ-decalactone productivity was obtained with OTR increase, with the highest value of 75 ± 10 mg L−1 h−1 being obtained in the STR for a kLa of 113 ± 5 h−1. However, a 2-fold increase in γ-decalactone concentration (around 3 g L−1) was achieved in the airlift compared to STR. Morphological characterization of the yeast cells by quantitative image analysis techniques showed that pneumatic agitation causes less impact in the cells morphology than mechanical agitation. A predominance of somewhat loose cells and quite irregular structures was observed in the STR. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/31632 |
DOI: | 10.1016/j.bej.2014.09.006 |
ISSN: | 1369-703X |
e-ISSN: | 1369-703X |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_18054_1.pdf | 1,29 MB | Adobe PDF | Ver/Abrir |