Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/31601
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Maciel, Marília de Holanda Cavalcanti | por |
dc.contributor.author | Ottoni, C. A. | por |
dc.contributor.author | Herculano, Polyanna N. | por |
dc.contributor.author | Porto, Tatiana Souza | por |
dc.contributor.author | Porto, Ana L. F. | por |
dc.contributor.author | Santos, Cledir | por |
dc.contributor.author | Lima, Nelson | por |
dc.contributor.author | Moreira, Keila | por |
dc.contributor.author | Motta, Cristina Maria de Souza | por |
dc.date.accessioned | 2014-12-03T19:12:13Z | - |
dc.date.available | 2014-12-03T19:12:13Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Maciel, M. H. C.; Ottoni, C. A.; Herculano, Polyanna N.; Porto, Tatiana S.; Porto, Ana L. F.; Santos, C.; Lima, Nelson; Moreira, Keila A.; Souza-Motta, Cristina, Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system. Fluid Phase Equilibria, 371, 125-130, 2014 | por |
dc.identifier.issn | 0378-3812 | por |
dc.identifier.uri | https://hdl.handle.net/1822/31601 | - |
dc.description.abstract | The partitioning and purification of polygalacturonases (PG) produced by Aspergillus niger URM 5162 were investigated in aqueous two-phase systems (ATPS), formed by polyethylene glycol and phosphate salts (PEG/phosphate). To evaluate the effect of the 4 independent variables – molar mass of polyethylene glycol (PEG) (400–8000 g/mol – MPEG), PEG concentration (12.5–17.5%, w/w – CPEG), phosphate concentration (15–25%, w/w, CPHOS) and pH (6.0–8.0) – on the 4 response variables: partition coefficient (K), activity yield (Y), purification factor (PF) and selectivity (S), a factorial design (24) was used. The endo-polygalacturonases (endo-PG) and exo-polygalacturonase (exo-PG) were preferentially partitioned in the top phase. For endo- and exo-PG, the highest values for the response variables K (1.23 and 2.40), Y (74.04% and 17.97%), PF (8.18 and 1.98) and S (24.68 and 48.07), respectively, were obtained for a CPEG of 12.5% (w/w), MPEG of 8000 g/mol, and CPHOS of 25% (w/w) at pH 6.0. These conditions were considered the most suitable for the purification of PG produced by A. niger URM 5162. Furthermore, the most important independent variables for endo- and exo-PG were CPHOS and MPEG, respectively. All independent variables studied and their interactions significantly influenced the response variables. According to these results, the PEG/phosphate system is a useful cost-effective alternative for purification of PG produced by A. niger URM 5162. | por |
dc.description.sponsorship | M.H.C. Maciel thanks to Conselho Nacional de Desenvolvimento Cientlfico e Tecnologico (CNPq, Brazil), Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE/APQ-0290-2.12/10), Financiadora de Estudos e Projetos (FINEP/ref. 2083/07) and Erasmus Mundus External Cooperation Window Lote 17. The authors thank the Laboratorio de Tecnologia de Bioativos (LABTECBIO), Federal Rural University of Pernambuco (Recife, Brazil). M.H.C. Maciel, C.A. Ottoni, C. Santos and N. Lima also thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013 and the Project "BioEnv - Biotechnology and Bioengineering for a sustainable world", REF. NORTE-07-0124-FEDER-000048." Co-funded by the Programa Operacional Regional do Norte (ON.2-O Novo Norte), QREN, FEDER. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier B.V. | por |
dc.rights | openAccess | por |
dc.subject | Aspergillus niger | por |
dc.subject | Partition | por |
dc.subject | PEG/phosphate ATPS | por |
dc.subject | Polygalacturonases | por |
dc.subject | Purification factor | por |
dc.title | Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.comments | CEB17752 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 125 | por |
oaire.citationEndPage | 130 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationTitle | Fluid Phase Equilibria | por |
oaire.citationVolume | 371 | por |
dc.date.updated | 2014-11-28T17:54:30Z | - |
dc.identifier.eissn | 0378-3812 | - |
dc.identifier.doi | 10.1016/j.fluid.2014.03.018 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Fluid Phase Equilibria | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_17752_1.pdf | 293,39 kB | Adobe PDF | Ver/Abrir |