Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/29314
Título: | Cellular uptake of multilayered capsules produced with natural and genetically engineered biomimetic macromolecules |
Autor(es): | Costa, Rui R. Girotti, Alessandra Santos, Mercedes Arias, F. J. Mano, J. F. Rodríguez-Cabello, José Carlos |
Palavras-chave: | Biomaterials Cellular uptake Layer-by-layer Multilayer capsules |
Data: | Fev-2014 |
Editora: | Elsevier 1 |
Revista: | Acta Biomaterialia |
Citação: | Costa R. R., Girotti A., Santos M., Arias F. J., Mano J. F., Rodríguez-Cabello J. C. Cellular Uptake of Multilayered Capsules Produced with Biomimetic and Genetically Engineered Macromolecules, Acta Biomaterialia, Vol. 10, Issue 6, pp. 2653-2662, doi:10.1016/j.actbio.2014.02.020, 2014 |
Resumo(s): | Multilayered microcapsules of chitosan and biomimetic elastin-like recombinamers (ELRs) were prepared envisaging the intracellular delivery of active agents. Two ELRs containing either a bioactive RGD sequence or a scrambled non-functional RDG were used to construct two types of functionalized polymeric microcapsules, both of spherical shape ∼4μm in diameter. Cell viability studies with human mesenchymal stem cells (hMSCs) were performed using microcapsule/cell ratios between 5:1 and 100:1. After 3 and 72h of co-incubation, no signs of cytotoxicity were found, but cells incubated with RGD-functionalized microcapsules exhibited higher viability values than RDG cells. The internalization efficacy and bioavailability of encapsulated DQ-ovalbumin were assessed by monitoring the fluorescence changes in the cargo. The data show that surface functionalization did not significantly influence internalization by hMSCs, but the bioavailability of DQ-ovalbumin degraded faster when encapsulated within RGD-functionalized microcapsules. The microcapsules developed show promise for intracellular drug delivery with increased drug efficacy. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/29314 |
DOI: | 10.1016/j.actbio.2014.02.020 |
ISSN: | 1742-7061 |
Versão da editora: | http://www.sciencedirect.com/science/article/pii/S1742706114000725# |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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17914-1-s2.0-S1742706114000725-main.pdf Acesso restrito! | 2,6 MB | Adobe PDF | Ver/Abrir |