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https://hdl.handle.net/1822/2607
Título: | Desorption of cellulases from cotton powder |
Autor(es): | Azevedo, Helena S. Ramos, Luiz Pereira Paulo, Artur Cavaco |
Palavras-chave: | Cellulases Cotton powder Degree of polymerisation Desorption Recovery Textile processing |
Data: | Set-2001 |
Editora: | Kluwer |
Revista: | Biotechnology Letters |
Citação: | "Biotechnology Letters". ISSN 0141-5492. 23:17 (Sept. 2001) 1445–1448. |
Resumo(s): | Cotton fabrics were treated with three different Trichoderma reesei cellulase preparations (total crude – TC, endoglucanase enriched – EG-rich, cellobiohydrolase enriched – CBH-rich) using mechanical agitation to produce cotton powder. Desorption of cellulase enzymes from the cotton powder was then performed by washing with buffer. After 3 washings most of the protein was desorbed from the cotton powder and the amount of sugars released in the latter washings was negligible. TC and CBH-rich preparations produced a finer cellulose powder than EGs. The desorption process caused a decrease in degree of polymerisation (DP) specially for the cotton treated with EGs and a marked increase in polydispersity (Pd ) for all preparations. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/2607 |
DOI: | 10.1023/A:1011619425613 |
ISSN: | 0141-5492 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals DET/2C2T - Artigos em revistas internacionais com arbitragem científica |