Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/25937
Título: | Evaluation of macroporous and microporous carriers for CHO-K1 cell growth and monoclonal antibody production |
Autor(es): | Rodrigues, E. Costa, A. R. Fernandes, Pedro Henriques, Mariana Cunnah, Philip Melton, David Azeredo, Joana Oliveira, Rosário |
Palavras-chave: | Monoclonal antibody Microcarrier Cytodex 3 CultiSpher-S Chinese hamster ovary cells |
Data: | 2013 |
Editora: | The Korean Society for Microbiology |
Revista: | Journal of Microbiology and Biotechnology |
Resumo(s): | The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monoclonal antibody (mAb) production by CHO-K1 cells. Cultures (5 mL) of microporous Cytodex 3 and macroporous CultiSpher-S carriers were performed in vented conical tubes and subsequently scaled-up (20 mL) to shake-flasks, testing combinations of different culture conditions (cell concentration, microcarrier concentration, rocking methodology, rocking speed and initial culture volume). Culture performance was evaluated considering mAb production and cell growth at the phases of initial adhesion and proliferation. The best culture performances were obtained with Cytodex 3, regarding cell proliferation (average 1.85 ± 0.11 x 106 cells/mL against 0.60 ± 0.08 x 106 cells/mL for CultiSpher-S), mAb production (2.04 ± 0.41 µg/mL against 0.99 ± 0.35 µg/mL for CultiSpher-S) and culture longevity (30 days against 10-15 days for CultiSpher-S), probably due to the collagen-coated dextran matrix that potentiates adhesion and prevents detachment. The culture conditions of greater influence were rocking mechanism (Cytodex 3, pulse followed by continuous) and initial cell concentration (CultiSpher-S, 4x105 cells/mL). Microcarriers proved to be a viable and favorable alternative to standard adherent and suspended cultures for mAb production by CHO-K1 cells, with simple operation, easy scale-up and significantly higher levels of mAb production. However, variations of microcarrier culture performance in different vessels reiterate the need for optimization at each step of the scale-up process. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/25937 |
DOI: | 10.4014/jmb.1304.04011 |
ISSN: | 1017-7825 1738-8872 |
Versão da editora: | http://www.jmb.or.kr/journal/viewJournal.html?year=2013&vol=23&num=9&page=1308 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Evaluation of macroporous and microporous carriers.pdf Acesso restrito! | Documento principal | 1,19 MB | Adobe PDF | Ver/Abrir |