Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/2576
Título: | Aspergillus niger β-galactosidase production by yeast in a continuous high cell density reactor |
Autor(es): | Domingues, Lucília Lima, Nelson Teixeira, J. A. |
Palavras-chave: | β-galactosidase production Continuous heterologous protein production Flocculation Whey permeate fermentation Recombinant yeast beta-galactosidase production |
Data: | Mar-2005 |
Editora: | Elsevier 1 |
Revista: | Process biochemistry |
Citação: | "Process biochemistry". ISSN 1359-5113. 40:3-4 (2005) 1151-1154. |
Resumo(s): | The continuous production of extracellular heterologous beta-galactosidase by a recombinant flocculating Saccharomyces cerevisiae, expressing the lacA gene (coding for beta-galactosidase) of Aspergillus niger was investigated. A continuous operation was run in a 6.51 airlift bioreactor with a concentric draft tube using lactose as substrate. Data on the operation with semi-synthetic medium with 50 and 100 g/l initial lactose concentrations are presented. The best result for beta-galactosidase productivity-6.2 x 10(5) U/l h-was obtained for a system operating at 0.24 h(-1) dilution rate and for a lactose concentration in the feed of 50 g/l. This value represents a 11-fold increase in beta-galactosidase productivity when compared to batch culture. Together with extracellular beta-galactosidase production an ethanol productivity of 9 g/l h was obtained for the bioreactor fed with 50 g/l initial lactose concentration at 0.45 h(-1) dilution rate. In addition to beta-galactosidase and ethanol production, this system allowed for complete lactose metabolism. The feasibility and advantages of using continuous high-cell-density systems operating with flocculent yeast cells for extracellular protein production is clearly shown. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/2576 |
DOI: | 10.1016/j.procbio.2004.04.016 |
ISSN: | 1359-5113 |
Versão da editora: | http://www.elsevier.com/wps/find/homepage.cws_home http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6THB-4CKFK62-5-9&_cdi=5278&_user=2459786&_orig=search&_coverDate=03%2F31%2F2005&_sk=999599996&view=c&wchp=dGLbVlz-zSkWA&md5=2718b52a36861d62b8315b422802c390&ie=/sdarticle.pdf |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DCILM - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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ProcessBiochemistry-Domingues[2].pdf | 78,42 kB | Adobe PDF | Ver/Abrir |