Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/25460
Título: | Batch and fed-batch growth of Pichia pastoris under increased air pressure |
Autor(es): | Lopes, Marlene Belo, Isabel Mota, M. |
Palavras-chave: | Pichia pastoris Increased air pressure Oxygen transfer rate (OTR) Crude glycerol |
Data: | 2013 |
Editora: | Springer Verlag |
Revista: | Bioprocess and Biosystems Engineering |
Resumo(s): | Pichia pastoris CBS 2612 behavior under air pressures of 1 bar, 3 bar and 5 bar in culture media of glycerol (pure and crude) and methanol was studied. Generally, the increase in oxygen transfer rate due to the increase of total pressure improved cellular growth for all carbon sources and for batch and fed-batch processes with different feeding rate strategies. In batch cultures, 1.4-fold, 1.2-fold, and 1.5-fold improvement in biomass production was obtained with the increase of air pressure up to 5 bar, using methanol, pure glycerol, and crude glycerol, respectively. The raise of air pressure to 5 bar using exponential feeding rate leaded to 1.4-fold improvement in biomass yield per glycerol mass consumed, for crude and pure glycerol. The current low cost of crude glycerol from the biodiesel production together with the present results shows the possibility of improving cell mass production of P. pastoris using increased air pressure. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/25460 |
DOI: | 10.1007/s00449-012-0871-5 |
ISSN: | 1615-7591 |
e-ISSN: | 1615-7605 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |