Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/25184
Título: | Development and characterization of PHB-HV based 3D scaffolds for a tissue engineering and cell-therapy combinatorial approach for spinal cord Injury regeneration |
Autor(es): | Samy, S. M. Silva, Nuno A. Correlo, Vitor M. Fraga, Joana S. Pinto, Luísa Teixeira-Castro, Andreia Leite-Almeida, Hugo Almeida, Armando Gimble, Jeffrey M. Sousa, Nuno Salgado, António J. Reis, R. L. |
Palavras-chave: | biocompatibility cell-based therapy PHB-HV 3D scaffolds spinal cord injury regeneration tissue engineering |
Data: | Ago-2013 |
Editora: | Wiley |
Revista: | Macromolecular Bioscience |
Citação: | Ribeiro-Samy S. M., Silva N. A., Correlo V. M., Fraga J. S., Pinto L. A., Teixeira-Castro A., Leite-Almeida H., Almeida A., Gimble J. M., Sousa N., Salgado A. J., Reis R. L. (2013). Development and Characterization of a PHB-HV-based 3D Scaffold for a Tissue Engineering and Cell-therapy Combinatorial Approach for Spinal Cord Injury Regeneration, Macromolecular Bioscience, doi:10.1002/mabi.201300178. |
Resumo(s): | Spinal cord injury (SCI) leads to devastating neurological deficits. Several tissue engineering (TE)- based approaches have been investigated for repairing this condition. Poly (3-hydroxybutyrateco- 3-hydroxyvalerate) (PHB-HV) is found to be particularly attractive for TE applications due to its properties, such as biodegradability, biocompatibility, thermoplasticity and piezoelectricity. Hence, this report addresses the development and characterization of PHB-HV-based 3D scaffolds, produced by freeze-drying, aimed to SCI treatment. The obtained scaffolds reveal an anisotropic morphology with a fully interconnected network of pores. In vitro studies demonstrate a lack of cytotoxic effect of PHB-HV scaffolds. Direct contact assays also reveal their ability to support the culture of CNS-derived cells and mesenchymal-like stem cells from different sources. Finally, histocompatibility studies show that PHB-HV scaffolds are well tolerated by the host tissue, and do not negatively impact the left hindlimb locomotor function recovery. Therefore results herein presented suggest that PHB-HV scaffolds may be suitable for SCI treatment. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/25184 |
DOI: | 10.1002/mabi.201300178 |
ISSN: | 1616-5195 |
Versão da editora: | https://onlinelibrary.wiley.com/doi/full/10.1002/mabi.201300178 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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ribeiro-samy s_macrom biosc 2013 posp.pdf | 1,73 MB | Adobe PDF | Ver/Abrir |