Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/2393| Título: | Studies of stabilization of native catalase using additives |
| Autor(es): | Costa, Silgia Tzanov, Tzanko Carneiro, Ana Filipa Gonçalves da Costa Paar, Andreas Gübitz, Georg M. Paulo, Artur Cavaco |
| Palavras-chave: | hydrogen peroxide catalase thermo and alkaline stability additives |
| Data: | Mar-2002 |
| Editora: | Elsevier 1 |
| Revista: | Enzyme and Microbial Technology |
| Citação: | "Enzyme and microbial technology". ISSN 0141-0229. 30:3 (Mar. 2002) 387–391. |
| Resumo(s): | Native catalase preparations isolated from Bacillus Sp were formulated with different additives for storage stabilization and better performance at high temperature and pH. The additives studied were: polyethylene glycol, glycerol, BSA, casein, glutaraldehyde, n-butylamine, ethylenediamine, 1.6-diaminohexane, BSA/glutaraldehyde and casein/glutaraldehyde. The glycerol and glutaraldehyde showed the best performance for long-term storage at 30degreesC and neutral pH. No stabilization additives were effective at pH 12, but below that pH the polyethylene glycol and glycerol appeared to be the most appropriate. Amines, polyethylene glycol and glycerol shifted the pH activity maximum of the native catalase toward more alkaline region, while glycerol were the only additive to improve the temperature profile of the enzyme. (C) 2002 Elsevier Science Inc. All rights reserved. |
| Tipo: | Artigo |
| URI: | https://hdl.handle.net/1822/2393 |
| DOI: | 10.1016/S0141-0229(01)00505-1 |
| ISSN: | 0141-0229 |
| Arbitragem científica: | yes |
| Acesso: | Acesso aberto |
| Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
