Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/22340
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Campo DC | Valor | Idioma |
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dc.contributor.author | Silva, Andreia Machado da | - |
dc.contributor.author | Tavares, Ana P. M. | - |
dc.contributor.author | Rocha, Cristina M. R. | - |
dc.contributor.author | Cristóvão, Raquel O. | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.contributor.author | Macedo, Eugénia A. | - |
dc.date.accessioned | 2013-01-07T17:55:04Z | - |
dc.date.available | 2013-01-07T17:55:04Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1359-5113 | por |
dc.identifier.uri | https://hdl.handle.net/1822/22340 | - |
dc.description.abstract | The aim of this work was to assess the possibility of using beer spent grain (a byproduct of beer’s brewing industry) as a carrier for laccase immobilization. Both adsorption (on spent grain – SG and on digested spent grain – DSG) and covalent binding (using glycidol and glycidol followed by ethylenediamine on DSG) were used. The effect of different immobilization conditions on the immobilization yields and recovered activities such as contact time, enzyme concentration and pH was evaluated. For the best conditions, immobilization yields, recovered activities and thermal, operational and storage stabilities were also evaluated. Finally, the Michaelis–Menten mechanism was applied and the parameter with respect to ABTS oxidation was determined. Enzyme immobilization on DSG led to the best enzyme activities (recovered activities as high as 90%) and to high storage and operational stabilities (10 cycles). Thermal stability was also improved and the half-life of immobilized laccase in SG increased from 0.64 h to 1.1 h at 70 ◦C | por |
dc.description.sponsorship | A.M. Silva thanks Fundacao para a Ciencia e Tecnologia (FCT, Portugal) for the Scholarship (BII/LAB/0020/2009). A.P.M. Tavares acknowledge the financial support (Programme Ciencia 2008) from FCT. This work is supported by projects PEst-/EQB/LA0020/2011 and PEst-/EQB/LA0006/2011, financed by FEDER through COMPETE - "Programa Operacional Fatores de Competitividade" and by FCT. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | openAccess | por |
dc.subject | Brewing | por |
dc.subject | Commercial laccase | por |
dc.subject | Immobilized enzyme | por |
dc.subject | Kinetic parameter | por |
dc.subject | Optimization | por |
dc.subject | Spent grain | por |
dc.title | Immobilization of commercial laccase on spent grain | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1095 | por |
oaire.citationEndPage | 1101 | por |
oaire.citationIssue | 7 | por |
oaire.citationTitle | Process Biochemistry | por |
oaire.citationVolume | 47 | por |
dc.identifier.doi | 10.1016/j.procbio.2012.03.021 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Process Biochemistry | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1-s2.0-S1359511312001353-main.pdf | 535,53 kB | Adobe PDF | Ver/Abrir |