Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/21326
Título: | Development of a short-term assay based on the evaluation of the plasma membrane integrity of the alga Pseudokirchneriella subcapitata |
Autor(es): | Machado, Manuela D. Soares, Eduardo V. |
Palavras-chave: | Membrane integrity Microplate assay Selenastrum capricornutum 1-Pentanol SYTOX green Acute toxicity |
Data: | 2012 |
Editora: | Springer |
Revista: | Applied Microbiology and Biotechnology |
Resumo(s): | Membrane integrity has been used as a criterion for the definition of cell viability. In the present work, staining conditions (time and dye concentration) for the evaluation of membrane integrity in a fluorescence microplate reader, using the membrane-impermeant nucleic-acid dye SYTOX Green, were optimized. Incubating Pseudokirchneriella subcapitata algal cells with 0.5 μmol/l SYTOX Green for 40 min allowed a clear discrimination between live (intact plasma membrane) and dead cells (with compromised plasma membrane). Algal cell suspensions, labelled with SYTOX Green, exhibited a green fluorescence proportional to the fraction of the cells with a permeabilized plasma membrane. The optimized staining conditions were used to assess the toxicity of 1-pentanol on P. subcapitata in a short-term exposure (6 h) assay. The loss of membrane integrity in the cell population increased with the concentration of 1-pentanol. The 6-h EC 10 and EC 50 values were 7,617 mg/l 1-pentanol (95 % confidence limits 4,670-9,327) and 12,818 mg/l 1-pentanol (95 % confidence limits 10,929-15,183), respectively. The developed microplate-based short-term assay can be useful in the high-throughput screening of toxics or environmental samples using the alga P. subcapitata |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/21326 |
DOI: | 10.1007/s00253-012-4185-y |
ISSN: | 0175-7598 (Print) |
Outros identificadores: | 1432-0614 (Online) |
Versão da editora: | http://dx.doi.org/10.1007/s00253-012-4185-y |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
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r.pdf Acesso restrito! | 364,76 kB | Adobe PDF | Ver/Abrir |