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https://hdl.handle.net/1822/1962
Título: | Production of 6-aminopenicillanic acid in aqueous two-phase systems by recombinant Escherichia coli with intracellular penicillin acylase |
Autor(es): | Cao, Xue-jun Wu, Xing-yan Fonseca, Luís Pina Cabral, J. M. S. Marcos, João Carlos |
Palavras-chave: | 6-aminopenicillanic acid production Aqueous two-phase systems Bioconversion Recombinant E. coli A56 Penicillin acylase |
Data: | 2004 |
Editora: | Kluwer |
Revista: | Biotechnology Letters |
Citação: | "Biotechnology letters". 26 (2004) 97-101. |
Resumo(s): | Bioconversion of Penicillin G in PEG 20000-Dextran T 70 aqueous two-phase systems was achieved using the recombinant Escherichia coli A56 (ppA22) with intracellular penicillin acylase as catalyst. The best conversion conditions were attained for: 7%(w/v) substrate (penicillin G), enzyme activity in bottom phase 52 U/ml, pH 7.8, temperature 37°C, reaction time 40 min. Five repeated batches could be performed in these conditions. Conversions ratios between 0.902-0.985mol of 6-aminopenicillanic acid (6-APA) per mol of penicillin G, were obtained and specific productivity was 3.6-4.6 μmol/min•ml. In addition the product 6-APA could directly be crystallized from the top phase with a purity of 96.2%. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/1962 |
DOI: | 10.1023/B:BILE.0000012885.62462.f3 |
ISSN: | 0141-5492 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CDQuim - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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ArtPenProd_RepUM.pdf | 157,68 kB | Adobe PDF | Ver/Abrir |