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https://hdl.handle.net/1822/17258
Título: | Quantitative proteomics (2D-DIGE) reveals molecular strategies employed by Burkholderia cenocepacia to adapt to the airways of cystic fibrosis patients under antimicrobial therapy |
Autor(es): | Madeira, Andreia Santos, P. M. Coutinho, Carla P. Oliveira, Ana Pinto de Correia, Isabel Sá |
Palavras-chave: | 2D-DIGE Burkholderia cenocepacia Antibiotic resistance Quantitative proteomics Chronic respiratory infections in cystic fibrosis Chronic respiratory infections in CF Microbiology |
Data: | Abr-2011 |
Editora: | Wiley |
Revista: | Proteomics |
Resumo(s): | Chronic respiratory infections caused by Burkholderia cenocepacia in patients with cystic fibrosis (CF) are characterized by low responsiveness to antibiotic therapy and, in general, to a more rapid decline of lung function. To get clues into the molecular mechanisms underlying the adaptive strategies employed to deal with the stressing conditions of the CF lung including antibiotic therapy, quantitative proteomics (2-D DIGE) was used to compare the expression programs of two clonal isolates retrieved from a chronically infected CF patient. Isolate IST439 was the first bacterium recovered while the clonal variant IST4113 was obtained after 3 years of persistent infection and intravenous therapy with ceftazidime/gentamicin. This isolate exhibits higher resistance levels towards different classes of antimicrobials. Proteins of the functional categories Energy metabolism, Translation, Nucleotide synthesis, Protein folding and stabilization are more abundant in IST4113, compared with IST439, suggesting an increased protein synthesis, DNA repair and stress resistance in IST4113. The level of proteins involved in peptidoglycan, membrane lipids and lipopolysaccharide synthesis is also altered and proteins involved in iron binding and transport are more abundant in IST4113. The quantitative comparison of the two proteomes suggests a genetic adaptation leading to increased antimicrobial resistance and bacterial persistence in the CF airways |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/17258 |
DOI: | 10.1002/pmic.201000457 |
ISSN: | 1615-9853 |
Versão da editora: | http://onlinelibrary.wiley.com/doi/10.1002/pmic.201000457/abstract |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Manuscripto_Proteomics.pdf Acesso restrito! | Documento principal | 1,28 MB | Adobe PDF | Ver/Abrir |