Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/16716
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Santos, Andréa F. S. | - |
dc.contributor.author | Carneiro-da-Cunha, Maria G. | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.contributor.author | Paiva, Patrícia M. G. | - |
dc.contributor.author | Coelho, L. C. B. B. | - |
dc.contributor.author | Nogueira, R. | - |
dc.date.accessioned | 2012-02-01T15:28:31Z | - |
dc.date.available | 2012-02-01T15:28:31Z | - |
dc.date.issued | 2011-07-04 | - |
dc.date.submitted | 2011-10-02 | - |
dc.identifier | 0366-6352 | por |
dc.identifier.issn | 0366-6352 | por |
dc.identifier.uri | https://hdl.handle.net/1822/16716 | - |
dc.description.abstract | The aim of this work was to characterise the affinity of protein preparations from Moringa oleifera seeds, specifically extract (seeds homogenised with 0.15 M NaCl), fraction (extract precipitated with 390 mg mL−1 of ammonium sulphate) and cMoL (coagulant M. oleifera lectin) to bind humic acids using a haemagglutinating activity assay with rabbit erythrocytes and a radial diffusion assay in agarose gel. Specific haemagglutinating activity (SHA) decreased by 94 % for the extract and cMoL and by 50 % for the fraction in the presence of humic acid. Precipitation bands were observed in the diffusion gel. Both results suggested humic acid-cMoL binding. Carbohydrates, potassium, and calcium ions and pH affected the SHA of cMoL. As an example of application, cMoL was immobilised on a column packed with sepharose receiving 20 mg mL−1 of carbon humic acid solution, 30 mg of humic acid per gram of support was removed. This result suggested that protein preparations might be used in water treatment to remove humic acids. | por |
dc.description.sponsorship | The authors express their gratitude to the Fundacao para Ciencia e a Tecnologia (FCT), the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), the VALNATURA ALFA Programme and the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) for their financial support. | por |
dc.language.iso | eng | por |
dc.publisher | Versita | por |
dc.rights | openAccess | por |
dc.subject | Humic acid | por |
dc.subject | Lectin | por |
dc.subject | Moringa oleifera | por |
dc.subject | Protein preparations | por |
dc.subject | Water treatment | por |
dc.title | Interaction of Moringa oleifera seed lectin with humic acid | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 406 | por |
oaire.citationEndPage | 411 | por |
oaire.citationIssue | 4 | por |
oaire.citationTitle | Chemical Papers | por |
oaire.citationVolume | 65 | por |
dc.identifier.doi | 10.2478/s11696-011-0025-2 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Chemical Papers | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |