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https://hdl.handle.net/1822/16712
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Campo DC | Valor | Idioma |
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dc.contributor.author | Pereira, Francisco B. | - |
dc.contributor.author | Guimarães, Pedro M. R. | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.contributor.author | Domingues, Lucília | - |
dc.date.accessioned | 2012-02-01T14:38:30Z | - |
dc.date.available | 2012-02-01T14:38:30Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1389-1723 | por |
dc.identifier.uri | https://hdl.handle.net/1822/16712 | - |
dc.description.abstract | The application and physiological background of two industrial Saccharomyces cerevisiae strains, isolated from harsh industrial environments, were studied in Very High Gravity (VHG) bio-ethanol fermentations. VHG laboratory fermentations, mimicking industrially relevant conditions, were performed with PE-2 and CA1185 industrial strains and the CEN.PK113-7D laboratory strain. The industrial isolates produced remarkable high ethanol titres (>19%, v/v) and accumulated an increased content of sterols (2 to 5-fold), glycogen (2 to 4-fold) and trehalose (1.1-fold), relatively to laboratory strain. For laboratory and industrial strains, a sharp decrease in the viability and trehalose concentration was observed above 90 g l-1 and 140 g l-1 ethanol, respectively. PE-2 and CA1185 industrial strains presented important physiological differences relatively to CEN.PK113-7D strain and showed to be more prepared to cope with VHG stresses. The identification of a critical ethanol concentration above which viability and trehalose concentration decrease significantly is of great importance to guide VHG process engineering strategies. This study contributes to the improvement of VHG processes by identifying yeast isolates and gathering yeast physiological information during the intensified fermentation process, which, besides elucidating important differences between these industrial and laboratory strains, can drive further process optimization. | por |
dc.description.sponsorship | The authors thank Daniel Gomes for performing some of the fermentation samples analyses, COPAM - Companhia Portuguesa de Amidos S.A. (Portugal) for kindly providing the CSL, and Rosane Schwan (Federal University of Lavras, Brazil) for kindly providing the yeast strains PE-2 and CA1185. The financial support of Fundacao para a Ciencia e a Tecnologia (FCT), Portugal, is acknowledged: project ProBioethanol PTDC/BIO/66151/2006, grant SFRH/BD/64776/2009 to F.B. Pereira and grant SFRH/BPD/44328/2008 to P.M.R. Guimaraes. | por |
dc.language.iso | eng | por |
dc.publisher | The Society for Biotechnology | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/66151/PT | - |
dc.rights | openAccess | por |
dc.subject | Bio-ethanol production | por |
dc.subject | Very high gravity fermentation | por |
dc.subject | Saccharomyces cerevisiae | por |
dc.subject | Industrial strains | por |
dc.subject | Stress tolerance | por |
dc.title | Robust industrial Saccharomyces cerevisiae strains for very high gravity bio-ethanol fermentations | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 130 | - |
oaire.citationEndPage | 136 | - |
oaire.citationIssue | 2 | - |
oaire.citationTitle | Journal of Bioscience and Bioengineering | por |
oaire.citationVolume | 112 | - |
dc.identifier.doi | 10.1016/j.jbiosc.2011.03.022 | por |
dc.identifier.pmid | 21543257 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Bioscience and Bioengineering | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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science2.pdf | 821,82 kB | Adobe PDF | Ver/Abrir |