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https://hdl.handle.net/1822/15873
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Campo DC | Valor | Idioma |
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dc.contributor.author | Conde, Artur | - |
dc.contributor.author | Silva, Paulo | - |
dc.contributor.author | Agasse, A. | - |
dc.contributor.author | Conde, Carlos | - |
dc.contributor.author | Gerós, H. | - |
dc.date.accessioned | 2011-12-27T11:15:43Z | - |
dc.date.available | 2011-12-27T11:15:43Z | - |
dc.date.issued | 2011-10 | - |
dc.date.submitted | 2011-08 | - |
dc.identifier.issn | 0032-0781 | por |
dc.identifier.uri | https://hdl.handle.net/1822/15873 | - |
dc.description | This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The definitive publisher-authenticated version is available online at http://pcp.oxfordjournals.org/cgi/content/abstract/pcr121? ijkey=6orgUM5fkIjedYn&keytype=ref | por |
dc.description.abstract | The intracellular accumulation of organic compatible solutes functioning as osmoprotectants, such as polyols, is an important response mechanism of several plants to drought and salinity. In Olea europaea a mannitol transport system (OeMaT1) was previously characterised as a key player in plant response to salinity. In the present study, heterotrophic sink models, such as olive cell suspensions and fruit tissues, and source leaves were used for analytical, biochemical and molecular studies. The kinetic parameters of mannitol dehydrogenase (MTD) determined in mannitol-growing cells, at 25 °C and pH 9.0, were as follows: Km, 54.5 mM mannitol and Vmax, 0.47 μmol h-1 mg-1 protein. The corresponding cDNA was cloned and named OeMTD1. OeMTD1 expression was correlated with MTD activity, OeMaT1 expression and carrier-mediated mannitol transport, in mannitol- and sucrose-growing cells. Furthermore, sucrosegrowing cells displayed only residual OeMTD activity, even though high levels of OeMTD1 transcription were observed. There is evidence OeMTD is regulated at both transcriptional and post-transcriptional levels. MTD activity and OeMTD1 expression were repressed after Na+, K+ and PEG treatments, both in mannitol- and sucrose-growing cells. In contrast, salt and drought significantly increased mannitol transport activity and OeMaT1 expression. Altogether, these studies support that olive tree copes with salinity and drought by coordinating mannitol transport with intracellular metabolism. | por |
dc.description.sponsorship | This work was supported by the Portuguese Foundation for Science and Technology (FCT) (research project ref. PTDC/AGR-ALI/100636/2008; to A. Conde, grant ref. SFRH/BD/47699/2008; to C. Conde, grant ref. SFRH/BPD/34998/2007; to P. Silvagrant ref. SFRH/BD/13460/2003). | por |
dc.language.iso | eng | por |
dc.publisher | Oxford University Press | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/100636/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F47699%2F2008/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F34998%2F2007/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F13460%2F2003/PT | - |
dc.rights | openAccess | por |
dc.subject | Environmental stress | por |
dc.subject | Mannitol | por |
dc.subject | Mannitol dehydrogenase | por |
dc.subject | Membrane transport | por |
dc.subject | Osmoprotectant | por |
dc.title | Mannitol transport and mannitol dehydrogenase activities are coordinated in olea europaea under salt and osmotic stresses | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | The original publication is available at http://pcp.oxfordjournals.org/content/early/2011/09/04/pcp.pcr121. | por |
sdum.publicationstatus | in publication | por |
oaire.citationStartPage | 1766 | por |
oaire.citationEndPage | 1775 | por |
oaire.citationIssue | 10 | por |
oaire.citationTitle | Plant and Cell Physiology | por |
oaire.citationVolume | 52 | por |
dc.identifier.doi | 10.1093/pcp/pcr121 | - |
dc.identifier.pmid | 21893515 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Plant and Cell Physiology | por |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Conde et al.2011OeMTD.pdf | Manuscript | 3,36 MB | Adobe PDF | Ver/Abrir |