Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/130
Título: | On-line simultaneous monitoring of glucose and acetate with FIA during high cell density fermentation of recombinant E. coli |
Autor(es): | Rocha, I. Ferreira, Eugénio C. |
Palavras-chave: | Escherichia coli Bioprocess monitoring Flow injection analysis Glucose Acetate On-line |
Data: | 2002 |
Editora: | Elsevier Science |
Revista: | Analytica Chimica Acta |
Citação: | "Analytica Chimica Acta". 462 (2002) 293–304. |
Resumo(s): | A two-channel flow injection analysis (FIA) system was developed for the simultaneous on-line monitoring of acetate and glucose during high cell density fed-batch fermentations of recombinant Escherichia coli. Acetate measurement was performed with a modified and optimised version of an existing method, based on acetate diffusion through a gas-diffusion chamber into a stream containing an acid–base indicator. The subsequent decrease in the absorbance was detected with an incorporated photometer. After method optimisation, it was possible to achieve linearity until 10 g/kg with no dilution step and with a detection level of 0.05 g/kg. Although some interferences were found, the performance of the method proved to be sufficiently reliable for on-line control purposes Commercially packed glucose oxidase (GOD) was used for the amperometric measurement of glucose. The method was linear up to 5 g/kg and it was possible to detect concentrations lower than 0.06 g/kg. For these measurements, no significant interferences were detected when the results were compared with other reference methods. The application of a simultaneous parallel configuration of the methods to a high cell density fed-batch E. coli fermentation was tested and reliable results were obtained within a 3 min delay. This information was made available to a supervisory computer running a developed LabVIEWTM programme via an Ethernet network, allowing the immediate implementation of control actions, improving the process performance. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/130 |
DOI: | 10.1016/S0003-2670(02)00347-1 |
ISSN: | 0003-2670 |
Versão da editora: | http://www.sciencedirect.com/science/article/pii/S0003267002003471 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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ACA2002.pdf | 301,37 kB | Adobe PDF | Ver/Abrir |