Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/11335
Título: | Improving bacterial cellulose for blood vessel replacement: functionalization with a chimeric protein containing a cellulose-binding module and an adhesion peptide |
Autor(es): | Andrade, Fábia K. Costa, Raquel Domingues, Lucília Soares, Raquel Gama, F. M. |
Palavras-chave: | Bacterial cellulose Cellulose-binding module Cell adhesion Endothelial cells Vascular grafts |
Data: | 2010 |
Editora: | Elsevier 1 |
Revista: | Acta Biomaterialia |
Citação: | "Acta Biomaterialia". ISSN 1742-7061. 6:10 (2010) 4034-4041. |
Resumo(s): | Chimeric proteins containing a cellulose-binding module (CBM) and an adhesion peptide (RGD or GRGDY) were produced and used to improve the adhesion of human microvascular endothelial cells (HMEC) to bacterial cellulose (BC). The effect of these proteins on the HMEC–BC interaction was studied. The results obtained demonstrated that recombinant proteins containing adhesion sequences were able to significantly increase the attachment of HMEC to BC surfaces, especially the RGD sequence. The images obtained by scanning electron microscopy showed that the cells on the RGD-treated BC present a more elongated morphology 48 h after cell seeding. The results also showed that RGD decreased the in-growth of HMEC cells through the BC and stimulated the early formation of cord-like structures by these endothelial cells. Thus, the use of recombinant proteins containing a CBM domain, with high affinity and specificity for cellulose surfaces allows control of the interaction of this material with cells. CBM may be combined with virtually any biologically active protein for the modification of cellulose-based materials, for in vitro or in vivo applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/11335 |
DOI: | 10.1016/j.actbio.2010.04.023 |
ISSN: | 1742-7061 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Andrade_Acta Biomaterialia.pdf | 915,48 kB | Adobe PDF | Ver/Abrir |