Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/10212
Título: | Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
Autor(es): | Santos, C. Paterson, R. R. M. Venâncio, Armando Lima, Nelson |
Palavras-chave: | Filamentous fungi Fungal identification MALDI-TOF ICMS Rapid techniques Spectral analysis |
Data: | Fev-2010 |
Editora: | Wiley-Blackwell |
Revista: | Journal of Applied Microbiology |
Citação: | "Journal of Applied Microbiology". ISSN 1364-5072. 108:2 (Febr. 2010) 375-385. |
Resumo(s): | Matrix-assisted laser desorption ⁄ ionization time-of-flight intact cell mass spectrometry (MALDI-TOF ICMS) is coming of age for the identification and characterization of fungi. The procedure has been used extensively with bacteria. UV-absorbing matrices function as energy mediators that transfer the absorbed photoenergy from an irradiation source to the surrounding sample molecules, resulting in minimum fragmentation. A surprisingly high number of fungal groups have been studied: (i) the terverticillate penicillia, (ii) aflatoxigenic, black and other aspergilli, (iii) Fusarium, (iv) Trichoderma, (iv) wood rotting fungi (e.g. Serpula lacrymans) and (v) dermatophytes. The technique has been suggested for optimizing quality control of fungal Chinese medicines (e.g. Cordyceps). MALDI-TOF ICMS offers advantages over PCR. The method is now used in taxonomic assessments (e.g. Trichoderma) as distinct from only strain characterization. Low and high molecular mass natural products (e.g. peptaibols) can be analysed. The procedure is rapid and requires minimal pretreatment. However, issues of reproducibility need to be addressed further in terms of strains of species tested and between run variability. More studies into the capabilities of MALDI-TOF ICMS to identify fungi are required. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/10212 |
DOI: | 10.1111/j.1365-2672.2009.04448.x |
ISSN: | 1364-5072 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DCILM - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Santos_JAM_MALDI paper.pdf | 151,22 kB | Adobe PDF | Ver/Abrir |